Effects of methanol and formic acid on CRYB, ALDH2, and ATP5A1 of RGCs

Figures & data

Table 1. RGCs group and deal with formic acid and methanol.

Sample	Group	N/mmol	Volume/μL	Sample	Group	N/mmol	Volume/μL
6-cm dish (48 h)
1	HCL Control	15	2.5	3	HCL Control	30	5
2	Formic acid	15	1.2	4	Formic Acid	30	2.4
6-cm dish (48 h)
5	Control	–	–	7	Methanol	30	2.4
6	Methanol	15	1.2
6-cm dish (7 d)
8	HCL Control	15	2.5	10	Control	–	–
9	Formic acid	15	1.2	11	Methanol	15	1.2

Table 2. Composition and procedure of RT-PCR.

Reagent	Volume/μL	Reaction condition
RNA	5 μg	70 °C, 5 min, centrifugation, and placed on ice shortly
Oligo (dT) 15 (10 μmol)	2
dNTP (2.5 mmol)	2
ddH2O (RNase-free)	Up to 14.5
All PCR tubes	14.5	Denaturation: 95 °C, 5 min Elongation: 42 °C, 60 min
5X RT buffer	4
HRP (RRI) RNase inhibitor	0.5
M-MLV	1
ddH2O (RNase-free)	Up to 20

Figure 1. RGCs stimulated with methanol and formic acid after 24 h: (A) Methanol group; (B) Formic acid group.

Figure 2. Expression of CRYB, ALDH2, and ATP5A1 with methanol and formic acid.

Figure 3. mRNA levels of CRYB, ALDH2, and ATP5A1 with methanol and formic acid.