The user safety assessment of a selenized yeast feed additive

Figures & data

Table 1. Acute skin irritation study: Test samples prepared and analysed.

Sample Name	Test System	Treatment	Amount per Well	Number of Replicates
Negative control	Live tissue	DPBS	20 µL	3
Positive control	Live tissue	5% (w/v) SDS	20 µL	3
Test item	Live tissue	SP3000	20 ± 2 mg	3
Test item without MTT (NSC living)	Live tissue	SP3000	20 ± 2 mg	2
Test item (NSMMT)	Killed tissue	SP3000	20 ± 2 mg	2
Negative control	Killed tissue	DPBS	20 µL	2
Test item without MTT (NSC killed)	Killed tissue	SP3000	20 ± 2 mg	2

Table 2. The optical density and viability results of the acute eye irritation study.

Test Item (n = 4)	Optical Density	Corrected Optical Density	Viability (%)
Blank Control	0.034
Negative Control (DPBS)	1.652	1.618	100
Positive Control (5% (w/v) SDS)	0.462	0.428	26.4
SP3000	1.441	1.407	86.9

Viability (%) = COD/COD Negative Control (alive tissues)*100.

Table 3. The optical density and viability results of the acute eye irritation study.

Test Item (n = 3)	Optical Density	Corrected Optical Density	Viability (%)
Blank Control	0.037	–	–
Negative Control (DPBS)	0.621	0.585	100
Positive Control (5% (w/v) SDS)	0.053	0.016	3
SP3000	0.612	0.575	98

Viability (%) = COD/COD Negative Control (alive tissues)*100.