Unio sp. primary cell culture potential in ecotoxicology research

Abstract

Primary tissue cultures from Unio pictorum were developed for utilization in ecotoxicology studies. Cytotoxic effects of selected plasticizers (BPA, DiNP, DiDP, DMP) were tested on digestive gland, gonad, gill and mantle cells. DiNP on gill and BPA on digestive gland were the highest and lowest cytotoxic compounds (IC50 0.111, 170.718 ppt). Mantle cells were the most suitable, reliable and easy to maintain cells; for toxicity assays. Unio primary cell culture provide valuable tool for testing/screening effects of xenobiotics in vitro, have potential for further development in terms of potential cryopreservation and stability comply with the 3 R principle.

Keywords:

• BPA
• In vitro ecotoxicology
• Phthalates
• Primary cell culture, Unio

Acknowledgement

Pınar Arslan was supported by TÜBİTAK under the 2211 – National Ph.D. Fellowship Programme 2015/1.