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Research Paper

Establishment of a high-throughput detection system for DNA demethylating agents

, , , , , , & show all
Pages 147-155 | Received 10 Aug 2016, Accepted 27 Nov 2016, Published online: 05 Mar 2018

Figures & data

Figure 1. Strategy to construct the detection system for DNA demethylating agents.

Figure 1. Strategy to construct the detection system for DNA demethylating agents.

Figure 2. Screening of appropriate transfectant clones.

Figure 2. Screening of appropriate transfectant clones.

Figure 3. Isolation of optimal epigenetic subclones from a transfectant clone.

Figure 3. Isolation of optimal epigenetic subclones from a transfectant clone.

Figure 4. High sensitivity of the subclone HML58-3 to 5-aza-dC determined by luminescence assay.

Figure 4. High sensitivity of the subclone HML58-3 to 5-aza-dC determined by luminescence assay.

Figure 5. Presence of nucleosome with inactive histone modification on the exogenous UCHL1 promoter.

Figure 5. Presence of nucleosome with inactive histone modification on the exogenous UCHL1 promoter.

Figure 6. Application of the subclone HML58-3 to HTS.

Figure 6. Application of the subclone HML58-3 to HTS.
Supplemental material

KEPI_A_1267887_supplementary_data.zip

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