Figures & data
Figure 1. Effect of PPT and DPN treatment on DNA methylation. (A) Decrease in global 5mC content in spermatozoa after DPN treatment. All values are expressed as means ± SEM. ** represents P < 0.01. (B) Methylation mapping of 15 CpG sites and total methylation of H19 DMR by pyrosequencing after PPT and DPN treatments. ‘a’ indicates P < 0.05, ‘b’ indicates P < 0.01, and ‘c’ indicates P < 0.001. n = 8.
Table 1. DNA methylation analysis of LINE-1 promoter by pyrosequencing after PPT and DPN treatments. Data represented as mean ± SEM n = 8. **P < 0.01; ***P < 0.001.
Figure 2. Effect of DNA methyltransferases in the testis after PPT and DPN treatments. (A) Transcript levels and (B) protein levels of Dnmts in testis after PPT and DPN treatments. (C) Representative images of protein bands of Dnmts and GAPDH. Results are expressed as means ± SEM. * indicates P < 0.05 and ** indicates P < 0.01. n = 8.
Figure 3. Effect of paternal PPT and DPN treatments on gene expression in resorbed embryos. (A) Expression of imprinted genes Igf2 and H19. (B) Expression of Dnmt1, Dnmt3a and Dnmt3b. ‘Control Res’ indicates resorbed embryos sired by control males; ‘PPT Res’ indicates resorbed embryos sired by PPT-treated males; ‘DPN Res’ indicates resorbed embryos sired by DPN-treated males. All the results are expressed as means ± SEM. * indicates P < 0.05. n = 8.
