A Comparison of the Predictive Power of DNA Methylation with Carbohydrate Deficient Transferrin for Heavy Alcohol Consumption

Figures & data

Figure 1. The distribution of carbohydrate-deficient transferrin (CDT, expressed as %) levels for HAC cases and controls

Figure 2. The distribution of ATS levels for HAC cases and controls

Table 1. Characteristics of study participants

	Case	Control
N	131 (41.8)	182 (58.2)
Age	41.8 ± 10.9	43.9 ± 14.9
Gender
Male	93 (71.0)	78 (42.9)
Female	38 (29.0)	104 (57.1)
Ethnicity
White, Non-Hispanic	110 (84.0)	165 (90.7)
African American	17 (13.0)	2 (1.1)
White, Hispanic	4 (3.0)	4 (2.2)
Other	0 (0.0)	11 (6.0)
Cotinine Serum Positive
Yes	114	6
No	17	176
THC Serum Positive
Yes	40	0
No	81	182
Drinks per day
Prior week	15.3 ± 10.1
Prior Month	16.0 ± 10.6
Prior 6 months	15.7 ± 11.2
Days since Last Drink	3.7 ± 1.8
Locus Methylation Values
cg02583484	16.6 ± 5.6 (%)	24.7 ± 4.8 (%)
cg04987734	43.7 ± 9.7 (%)	32.2 ± 6.8 (%)
cg09935388	59.3 ± 18.9 (%)	81.7 ± 11.0 (%)
cg04583842	45.6 ± 7.1 (%)	37.5 ± 5.5 (%)
ATS	6.73 ± 4.07	0.04 ± 2.21
CDT	2.66 ± 3.31 (%)	0.62±0.19 (%)

Alcohol methylation T-score (ATS), carbohydrate-deficient transferrin (CDT).

Table 2. CDT and ATS comparison by sex for HAC cases and controls

		Case		Control
CDT	Female Mean (SD)	1.79 (2.29)	p = 0.02	0.63 (0.20)	p = 0.33
	Male Mean (SD)	3.01 (3.60)		0.60 (0.18)
ATS	Female Mean (SD)	6.99 (4.44)	p = 0.65	0.18 (2.30)	p = 0.34
	Male Mean (SD)	6.63 (3.93)		−0.14 (2.07)

CDT is expressed in per cent (%).

Table 3. AUC and AIC for Logistic Regression of CDT and ATS Values for Predicting HAC

Model	Predictors			AUC		AIC	Aggregated AUC^†
1	Gender + Age			0.649		405	0.612
2	Gender + Age + CDT		0.865	0.865	267	267	0.840
3	Gender + Age + ATS		0.959	0.959	167	167	0.944
4	Gender + Age + ATS + CDT	0.977		0.977		126	0.950

†Estimated from 200 bootstrap samples.

Figure 3. Receiver Operator Characteristic Curve (ROC) for CDT

Figure 4. Receiver Operator Characteristic Curve (ROC) for ATS

Figure 5. Aggregated receiver operator characteristic curve (ROC) for CDT. The dotted grey lines are the ROC curves for the bootstrap samples and the solid black line is the aggregated ROC curve

Figure 6. Aggregated receiver operator characteristic curve (ROC) for ATS. The dotted grey lines are the ROC curves for the bootstrap samples and the solid black line is the aggregated ROC curve