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Epigenetics Volume 17, 2022 - Issue 10
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Brief Report

Direct bisulphite conversion of cervical samples for DNA methylation analysis

Lisanne Verhoefa Amsterdam UMC, Vrije Universiteit Amsterdam, Pathology, Cancer Center Amsterdam, De BoelelaanAmsterdam, The NetherlandsORCID Iconhttps://orcid.org/0000-0001-9877-0297View further author information
ORCID Icon,
A. N Flooreb Self-screen B.v., Amsterdam, The NetherlandsORCID Iconhttps://orcid.org/0000-0002-5835-2688View further author information
ORCID Icon,
Saskia Doornb Self-screen B.v., Amsterdam, The NetherlandsView further author information
,
Kate Cuschieric Scottish Human Papilloma Virus Reference Laboratory, Royal Infirmary of Edinburgh, Edinburgh, UKORCID Iconhttps://orcid.org/0000-0002-2604-3928View further author information
ORCID Icon,
Ramya Bhatiac Scottish Human Papilloma Virus Reference Laboratory, Royal Infirmary of Edinburgh, Edinburgh, UK;d Centre for Reproductive Health, University of Edinburgh, Edinburgh, UKORCID Iconhttps://orcid.org/0000-0001-5294-0668View further author information
ORCID Icon,
A. T Hesselinkb Self-screen B.v., Amsterdam, The NetherlandsView further author information
,
Chris J.L.M. Meijera Amsterdam UMC, Vrije Universiteit Amsterdam, Pathology, Cancer Center Amsterdam, De BoelelaanAmsterdam, The NetherlandsORCID Iconhttps://orcid.org/0000-0002-2758-9463View further author information
ORCID Icon,
Renske D.M. Steenbergena Amsterdam UMC, Vrije Universiteit Amsterdam, Pathology, Cancer Center Amsterdam, De BoelelaanAmsterdam, The NetherlandsORCID Iconhttps://orcid.org/0000-0002-2327-9839View further author information
ORCID Icon &
Daniëlle A.M. Heidemana Amsterdam UMC, Vrije Universiteit Amsterdam, Pathology, Cancer Center Amsterdam, De BoelelaanAmsterdam, The NetherlandsCorrespondence[email protected]
ORCID Iconhttps://orcid.org/0000-0001-6463-7391View further author information
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Pages 1173-1179 | Received 02 Mar 2021, Accepted 08 Oct 2021, Published online: 27 Oct 2021

Figures & data

Figure 1. Flowchart of the protocols used.

Figure 1. Flowchart of the protocols used.

Figure 2. Correlation of ΔΔCq values of FAM19A4 (a) and miR124-2 (b) between direct cell conversion and reference protocol. For both x- and y-axis: a lower ΔΔCq value represents a higher methylation value. Bland–Altman plot for FAM19A4 (c) and miR124-2 (d). The horizontal red line shows the mean of the difference in ΔΔCq value (=bias) between the two protocols, being 0.167 for FAM19A4 and −0.142 for miR124-2. The horizontal green lines show the upper and lower 95% limits of agreement (= bias ±1.96 × SD), being −2.274 and +2.608, respectively, for FAM19A4 and −2.316 and +2.031, respectively, for miR124-2.

Figure 2. Correlation of ΔΔCq values of FAM19A4 (a) and miR124-2 (b) between direct cell conversion and reference protocol. For both x- and y-axis: a lower ΔΔCq value represents a higher methylation value. Bland–Altman plot for FAM19A4 (c) and miR124-2 (d). The horizontal red line shows the mean of the difference in ΔΔCq value (=bias) between the two protocols, being 0.167 for FAM19A4 and −0.142 for miR124-2. The horizontal green lines show the upper and lower 95% limits of agreement (= bias ±1.96 × SD), being −2.274 and +2.608, respectively, for FAM19A4 and −2.316 and +2.031, respectively, for miR124-2.

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