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Epigenetics Volume 17, 2022 - Issue 13
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Brief Report

Evaluating DNA recovery efficiency following bisulphite modification from plasma samples submitted for cell-free DNA methylation analysis

Daniel RA Coxa Department of Surgery (Austin Precinct), The University of Melbourne, Heidelberg, Australia;b Translational Genomics and Epigenomics Laboratory, Department of Surgery (Austin Precinct), The University of Melbourne, Heidelberg, Australia;c Hepatopancreatobiliary and Transplant Surgery Unit, Austin Health, Heidelberg, AustraliaORCID Iconhttps://orcid.org/0000-0002-5092-4370View further author information
ORCID Icon,
Boris Ka Leong Wongb Translational Genomics and Epigenomics Laboratory, Department of Surgery (Austin Precinct), The University of Melbourne, Heidelberg, AustraliaView further author information
,
Eunice Leea Department of Surgery (Austin Precinct), The University of Melbourne, Heidelberg, Australia;c Hepatopancreatobiliary and Transplant Surgery Unit, Austin Health, Heidelberg, AustraliaView further author information
,
Adam Testrod Liver Transplant Unit, Austin Health, Heidelberg, AustraliaView further author information
,
Vijayaragavan Muralidharana Department of Surgery (Austin Precinct), The University of Melbourne, Heidelberg, Australia;c Hepatopancreatobiliary and Transplant Surgery Unit, Austin Health, Heidelberg, AustraliaView further author information
,
Alexander Dobrovica Department of Surgery (Austin Precinct), The University of Melbourne, Heidelberg, Australia;b Translational Genomics and Epigenomics Laboratory, Department of Surgery (Austin Precinct), The University of Melbourne, Heidelberg, AustraliaView further author information
&
Su Kah Goha Department of Surgery (Austin Precinct), The University of Melbourne, Heidelberg, Australia;c Hepatopancreatobiliary and Transplant Surgery Unit, Austin Health, Heidelberg, AustraliaCorrespondence[email protected] [email protected]
ORCID Iconhttps://orcid.org/0000-0002-6684-2521View further author information
ORCID Icon show all
Pages 1956-1960 | Received 22 Nov 2021, Accepted 15 Jun 2022, Published online: 28 Jun 2022

Figures & data

Figure 1. Control chart illustrating cfDNA recovery in 107 consecutive patient samples following cfDNA extraction and bisulphite modification as measured by ddPCR using CEREBIS. The mean recovery efficiency was 37% ± 6. Upper and lower control limits (UCL, LCL) were set according to the beta probability distribution. Samples breaching control limits (n = 9) are marked with an X. cfDNA extraction and bisulphite modification batches are marked.

Figure 1. Control chart illustrating cfDNA recovery in 107 consecutive patient samples following cfDNA extraction and bisulphite modification as measured by ddPCR using CEREBIS. The mean recovery efficiency was 37% ± 6. Upper and lower control limits (UCL, LCL) were set according to the beta probability distribution. Samples breaching control limits (n = 9) are marked with an X. cfDNA extraction and bisulphite modification batches are marked.
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Data availability statement

The data that support the findings of this study are available from the corresponding author on application upon reasonable request.

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