Figures & data
Figure 1. (a) Domain structure of the dCas9–dnmt7/Tet2 catalytic domain fusion protein. The nuclease inactivating mutations D10A and H840A of Cas9 are indicated. Deactivated Cas9 was fused to the catalytic domain of the zebrafish de novo DNA methyltransferase 7 (Dnmt7CD) or ten-eleven translocation 2 (Tet2CD) using a short Gly4Ser peptide (GS); (b) Illustration of the locations of the three gRNAs designed to target the upstream regions of the dmrt1 coding sequence; (c) Average DNA methylation level of target locus within dmrt1 TSS region (Two-way ANOVA test); (d) Methylation levels of individual CpGs in the TSS region of the dmrt1 gene. The asterisk indicates statistical significance. (* p<0.05).
Figure 2. (a) Illustration of the locations of the gRNA designed for targeting the TSS region of the cyp19a1a gene; (b) Methylation levels of individual CpGs in the TSS region of the cyp19a1a gene; (c) The average DNA methylation level of target locus within the dmrt1 TSS region at 24 hpf. (d) Methylation levels of individual CpGs in the TSS region of the dmrt1 gene at 24 hpf. (e) The average DNA methylation level of target locus within dmrt1tss region at 6 hpf. (f) Methylation levels of individual CpGs in the TSS region of the dmrt1 gene at 6 hpf. The asterisk indicates statistical significance. (* p<0.05).
Supplemental Material
Download MS Word (402.2 KB)Data availability statement
The sequence data generated in this study is available at the Genome Sequence Archive (GSA), under accession number CRA006715, which is accessible at https://ngdc.cncb.ac.cn/gsa.