Identification of differentially methylated genes for severe acne by genome-wide DNA methylation and gene expression analysis

Figures & data

Figure 1. Quantified the cellular composition of peripheral blood cells. (a) Six immune cells of peripheral blood in stage I. (b) Six immune cells of peripheral blood in stage II.

Figure 2. Differences in six immune cells in blood were compared between patients and healthy controls. (a) Differences in stage I. (b) Differences in stage II.

Figure 3. QQ plot to assess whether the epigenome-wide association results were robust to analysis on M-values instead of β-values.

Table 1. Meta-analysis results of differential methylation sites of severe acne in stage I and stage II.

ID	Chr	Bp	DeltaBeta	pVal.fixed	pValQ	Direction	pVal.final	FDR	Gene
cg03020863	11	104005644	−0.09	1.70E–08	6.89E–01	–	1.70E–08	6.82E–07	PDGFD
cg19214707	7	3157722	0.24	2.73E–07	7.37E–01	++	2.73E–07	5.45E–06	LOC105375130
cg24833027	8	1897969	−0.07	1.64E–06	7.20E–01	–	1.64E–06	2.19E–05	ARHGEF10
cg27154217	5	50023127	0.08	9.30E–06	3.33E–01	++	9.30E–06	9.30E–05	PARP8
cg01479768	2	731298	−0.06	1.43E–05	6.48E–01	–	1.43E–05	1.15E–04	LOC105373358
cg03192273	5	150618948	0.14	1.12E–04	6.41E–01	++	1.12E–04	7.48E–04
cg11102724	5	150619039	0.15	1.36E–04	6.39E–01	++	1.36E–04	7.75E–04
cg18946787	12	133052221	0.05	2.29E–04	8.05E–01	++	2.29E–04	1.02E–03	MUC8
cg13102742	17	35208812	−0.24	2.30E–04	7.00E–01	–	2.30E–04	1.02E–03
cg19964325	2	102690795	−0.06	2.98E–04	5.19E–01	–	2.98E–04	1.11E–03	IL1R1
cg20652636	2	85656009	0.07	3.12E–04	6.19E–01	++	3.12E–04	1.11E–03	SH2D6
cg07161061	12	133057193	−0.09	3.34E–04	9.60E–01	–	3.34E–04	1.11E–03
cg20363450	1	201540458	0.07	3.69E–04	3.43E–01	++	3.69E–04	1.11E–03	NAV1
cg03249723	9	98880057	−0.10	3.89E–04	9.37E–01	–	3.89E–04	1.11E–03	LOC158434
cg01741372	11	783889	−0.07	5.14E–04	7.29E–01	–	5.14E–04	1.37E–03	LOC171391
cg23471274	19	43955029	0.06	7.35E–04	8.57E–01	++	7.35E–04	1.84E–03
cg12869809	1	206904547	−0.07	8.70E–04	8.34E–01	–	8.70E–04	2.05E–03	MAPKAPK2
cg12114963	1	228122362	0.06	1.15E–03	9.38E–01	++	1.15E–03	2.55E–03	WNT9A
cg04345744	1	196507743	0.06	1.43E–03	9.81E–01	++	1.43E–03	3.00E–03	KCNT2
cg09533556	10	64441361	−0.09	1.77E–03	4.43E–01	–	1.77E–03	3.54E–03
cg08461876	1	59437584	0.07	2.31E–03	7.56E–01	++	2.31E–03	4.41E–03	LINC02777
cg03461641	4	26454190	−0.09	3.02E–03	6.54E–01	–	3.02E–03	5.48E–03
cg12313868	11	30338751	−0.11	3.36E–03	6.85E–01	–	3.36E–03	5.85E–03	LOC105376607

Data availability statement

Datasets related to this article have been deposited in the OMIX, China National Center for Bioinformation/Beijing Institute of Genomics, Chinese Academy of Sciences (https://ngdc.cncb.ac.cn/omix: accession no. OMIX704, and OMIX706).