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Research Article

Chronic intermittent ethanol exposure-induced m6A modifications around mRNA stop codons of opioid receptor genes

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Article: 2294515 | Received 05 Aug 2023, Accepted 05 Dec 2023, Published online: 20 Dec 2023

Figures & data

Figure 1. Chronic intermittent ethanol (CIE) exposure/withdrawal-induced global m6A RNA methylation changes. CTL: Control SH-SY5Y or SW620 cells (without ethanol exposure). CIE: a 3-week chronic intermittent ethanol (CIE) exposure. CIE+WD: a 3-week chronic intermittent ethanol exposure followed a 24-hr ethanol withdrawal.

Figure 1. Chronic intermittent ethanol (CIE) exposure/withdrawal-induced global m6A RNA methylation changes. CTL: Control SH-SY5Y or SW620 cells (without ethanol exposure). CIE: a 3-week chronic intermittent ethanol (CIE) exposure. CIE+WD: a 3-week chronic intermittent ethanol exposure followed a 24-hr ethanol withdrawal.

Figure 2. Chronic intermittent ethanol (CIE) exposure/withdrawal-induced mRNA methylation and expression changes in the μ-opioid receptor gene (OPRM1). CTL: Control SH-SY5Y or SW620 cells (without ethanol exposure). CIE: a 3-week chronic intermittent ethanol (CIE) exposure. CIE+WD: a 3-week chronic intermittent ethanol exposure followed a 24-hr ethanol withdrawal.

Figure 2. Chronic intermittent ethanol (CIE) exposure/withdrawal-induced mRNA methylation and expression changes in the μ-opioid receptor gene (OPRM1). CTL: Control SH-SY5Y or SW620 cells (without ethanol exposure). CIE: a 3-week chronic intermittent ethanol (CIE) exposure. CIE+WD: a 3-week chronic intermittent ethanol exposure followed a 24-hr ethanol withdrawal.

Figure 3. Chronic intermittent ethanol (CIE) exposure/withdrawal-induced mRNA methylation and expression changes in the δ-opioid receptor gene (OPRD1). CTL: Control SH-SY5Y or SW620 cells (without ethanol exposure). CIE: a 3-week chronic intermittent ethanol (CIE) exposure. CIE+WD: a 3-week chronic intermittent ethanol exposure followed a 24-hr ethanol withdrawal.

Figure 3. Chronic intermittent ethanol (CIE) exposure/withdrawal-induced mRNA methylation and expression changes in the δ-opioid receptor gene (OPRD1). CTL: Control SH-SY5Y or SW620 cells (without ethanol exposure). CIE: a 3-week chronic intermittent ethanol (CIE) exposure. CIE+WD: a 3-week chronic intermittent ethanol exposure followed a 24-hr ethanol withdrawal.

Figure 4. Chronic intermittent ethanol (CIE) exposure/withdrawal-induced mRNA methylation and expression changes in the κ-opioid receptor gene (OPRK1). CTL: Control SH-SY5Y or SW620 cells (without ethanol exposure). CIE: a 3-week chronic intermittent ethanol (CIE) exposure. CIE+WD: a 3-week chronic intermittent ethanol exposure followed a 24-hr ethanol withdrawal.

Figure 4. Chronic intermittent ethanol (CIE) exposure/withdrawal-induced mRNA methylation and expression changes in the κ-opioid receptor gene (OPRK1). CTL: Control SH-SY5Y or SW620 cells (without ethanol exposure). CIE: a 3-week chronic intermittent ethanol (CIE) exposure. CIE+WD: a 3-week chronic intermittent ethanol exposure followed a 24-hr ethanol withdrawal.
Supplemental material

Supplementary Materials 20231118.docx

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Data availability statement

The data that support the findings of this study are available upon request from the corresponding author, HZ.