Spatio-temporal changes in endogenous abscisic acid contents during etiolated growth and photomorphogenesis in tomato seedlings

Figures & data

Figure 1. Spatiotemporal changes in ABA content presented in particular organs of tomato seedlings. ABA concentrations during germination in the D (72h) and etiolated growth in the D and during de-etiolation and photomorphogensis in BL (96 h-120 h) are visualized as a “heat-map” by color range. The colors represent median values of absolute ABA concentrations in given part of the seedling that were calculated from 3 biological repeats and correspond to the scale (0 – 80 pmol/g of fresh weight - FW).

Table 1. Quantification of free ABA in particular plant organs of tomato seedlings grown in the dark and under blue light. The data are presented as medians of 3 biological experiments, statistical significance was performed using Mann-Whitney test.

Time	Organ	DARK ABA [pmol/g FW]	BLUE LIGHT ABA [pmol/g FW]	p value
96 h	Cotyledon	25.27	19.37	<0.01
	Hypocotyl	28.44	17.86	<0.01
	Root	11.70	9.98	<0.07
120 h	Cotyledon	77.45	41.33	<0.01
	Hypocotyl	33.76	22.54	<0.01
	Root	18.26	8.87	<0.01

Figure 2. Hypothetical model of ABA action in etiolated growth of tomato seedlings. The abscisic acid content is reduced by BL exposure to promote de-etiolation. When the seedlings are kept in the D, ABA inhibits maturation of chloroplasts and development of stomata, but supports the cell elongation to maintain hypocotyl growth. (The part of the figure was adopted from Wikipedia free illustrations under CC license).