Characterization of SCL33 splicing patterns during diverse virus infections in Brachypodium distachyon

Figures & data

Table 1. Sequence composition of Bd-SCL33 splice variants in Brachypodium^a

Transcript ID	Length (nt)	AS pattern	Predicted protein (aa)
7	3048	Full-length pre-mRNA	9
6	2417	Intron III retention (nt 1–496) + Intron I retention (nt 1–872)	9
5	1545	Intron III retention (nt 1–496)	75
4	1315	Intron III retention (nt 231–496)	78
3	1157	Intron III retention (nt 231–338)	78
2	1049	AS transcript (Bradi1g61330.2)	219
1	1135	Primary transcript (Bradi1g61330.1)	246

^a Modified from Mandadi and Scholthof, 2015, Plant Cell 27:71-85.

Figure 1. Bd-SCL33 differential splicing during diverse grass virus infections. Expression of Bd-SCL33 transcripts in mock, PMV+SPMV-, BMV-, BSMV-, MMMV-, SYBV-, WSMV- and FoMV-infected Brachypodium at 10, 21 and 42 days postinoculation (dpi), as determined by RT-PCR. The alternatively spliced Bd-SCL33 transcripts, as defined in Table 1, are identified by an asterisk (*). UBC18 was used as control for approximately equal cDNA amounts in the different samples. Lane “M” indicates DNA molecular weight markers.