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Article Addendum

Requirement of MIR165A primary transcript sequence for its activity pattern in Arabidopsis leaf primordia

, &
Article: e1055432 | Received 15 May 2015, Accepted 22 May 2015, Published online: 31 Aug 2015

Figures & data

Figure 1. (A) Primary transcripts of MIR165A and 3' end-truncated MIR165A. Red and orange boxes indicates miR165 and miR165a*, respectively. Gray boxes represent pri-miR165a. A dashed-line box indicate the lack of the 3'-region in 3' end-truncated MIR165A. (B) Schematic model of the activity patterns of miR165 arisen from the MIR165A (left) and 3' end-truncated MIR165A (right) in leaf primordia. Black color indicates the MIR165A-expressing and miR165-active cell layer, and gray color represents miR165-active cell layers.

Figure 1. (A) Primary transcripts of MIR165A and 3' end-truncated MIR165A. Red and orange boxes indicates miR165 and miR165a*, respectively. Gray boxes represent pri-miR165a. A dashed-line box indicate the lack of the 3'-region in 3' end-truncated MIR165A. (B) Schematic model of the activity patterns of miR165 arisen from the MIR165A (left) and 3' end-truncated MIR165A (right) in leaf primordia. Black color indicates the MIR165A-expressing and miR165-active cell layer, and gray color represents miR165-active cell layers.

Figure 2. Schematic model of a possible scenario for the confinement of miR165 activity pattern dependent on the MIR165A primary transcript (pri-miR165a) in leaf primordia. In the abaxial epidermal cells, a part of pri-miR165a is exported from the nucleus to the cytoplasm (blue arrow), and Factor-X binds to the 3' end-region of the pri-miR165a. The pri-miR165a/Factor-X complex moves to the abaxial L2 and L3 cells through plasmodesmata (green arrow). In the abaxial L2 and L3 cells, the pri-miR165a/Factor-X complex is imported into the nucleus (red arrow), and the pri-miR165a is dissected by DCL1 in the nucleus.

Figure 2. Schematic model of a possible scenario for the confinement of miR165 activity pattern dependent on the MIR165A primary transcript (pri-miR165a) in leaf primordia. In the abaxial epidermal cells, a part of pri-miR165a is exported from the nucleus to the cytoplasm (blue arrow), and Factor-X binds to the 3' end-region of the pri-miR165a. The pri-miR165a/Factor-X complex moves to the abaxial L2 and L3 cells through plasmodesmata (green arrow). In the abaxial L2 and L3 cells, the pri-miR165a/Factor-X complex is imported into the nucleus (red arrow), and the pri-miR165a is dissected by DCL1 in the nucleus.

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