Cytosolic ppGpp accumulation induces retarded plant growth and development

Figures & data

Figure 1. ppGpp overaccumulation results in retarded growth of Arabidopsis. The yjbM gene¹³ was synthesized in vitro with adapting its codon usage for expression in Arabidopsis. The DNA fragment was cloned into the estrogen-inducible expression vector pER8 at its SpeI and XhoI sites.¹⁵ The resulting plasmid was introduced into Arabidopsis wild type (ecotype Columbia) by the Agrobacterium transformation method,¹⁶ and the obtained mutant was designated as YJBMox. As a control, the empty vector (pER8) was also introduced. (A) Plants grown in MS medium for 10 days were transferred to the MS medium containing 2 μM β-estradiol, and ppGpp levels in each line were quantified after 0, 1, 3, and 10-days. (B) Phenotypes of plants 0 and 10 days after the transfer.

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