Figures & data
Figure 1. Sequence structure of CB5C and mutants. a) Gene structure of A. thaliana CB5C, showing the T-DNA insertion sites for the 2 mutants (cb5c-1 and cb5c-2). Black boxes depict exons; white boxes indicate introns and untranslated regions. b) Sequence comparison between the protein products of WT and cb5c-1. The N-terminal heme-binding domain (gray), the C-terminal transmembrane helix (black) and the additional domain of the cb5c-1 mutant.
Figure 2. Expression levels of CB5C. The three genotypes (WT, cb5c-1 and cb5c-2) were analyzed for CB5C expression. Bar graphs display the mean expression (normalized to WT levels) and error-bars display the standard deviation of 3 independent biological replicates for each genotype.
Table 1. Genes co-expressing with A. thaliana CB5C
Table 2. Glucosinolate levels in seedlings of the cb5c mutants (nmol/g FW).
Figure 3. Biomass response to MeJA. Biomass responses of 10-day-old seedlings of the 3 genotypes (WT, cb5c-1 and cb5c-2). The weight of seedlings treated with methyl-jasmonate (MeJA) was normalized to the weight of untreated seedlings (ctrl). Different letters refer to statistical groupings (pairwise t-test as post-hoc, p < 0.05, n = 30). See Table S1 for the ANOVA table.
