http://orcid.org/0000-0001-5356-4350
Figures & data
Figure 1. The columella root cap marker PET111 has similar expression in both Col-0 and nlp7-1. (A) PET111 expression in Col-0, (B) PET111 X nlp7-1. Scale bar = 100 µm.
Figure 2. NLP7 does not regulate the gravity sensing mechanism of the root cap. (A) Measurement of root curvature angle 24 h after gravity treatment in WT and nlp7-1 mutant plants. No significant difference (P > 0.05) was observed between WT and nlp7-1. N = 18 or more roots. (B) and (C) Root caps of WT (B) and nlp7-1 (C) stained with 1% Lugol's solution to stain for starch. Images taken at 100X. Scale bar = 20 µm.
Figure 3. CEL3 is expression is upregulated in nlp7-1 mutant but mutations in CEL3 do not lead to a sticky root cap layer phenotype. (A) Quantitative PCR showing upregulation of CEL3 in nlp7-1 mutant. Error bars show standard deviation between three independent biological replicates. P < 0.05, (B) Number of root cap layers from columella initials to the last layer of border like cells. No significant difference was observed in the number of root cap layers between WT and the cel3-1 mutant. N = 20 roots per genotype.
