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Short Communication

AUXIN RESPONSE FACTOR3 plays distinct role during early flower development

, , ORCID Icon, ORCID Icon &
Article: e1467690 | Received 01 Apr 2018, Accepted 18 Apr 2018, Published online: 26 Jun 2018

Figures & data

Figure 1. Floral meristem (FM) determinacy and organ polarity phenotype of Ler (A), arf3-29 (B), kan1 kan2 (C), ag-10 (D), ag-10 arf3-29 (E) and ag-10kan1 kan2 (F). ag-10 arf3-29 displayed FM indeterminacy phenotype showing bulged gynoecium (marked by red arrow). kan1 kan2 and ag-10 kan1 kan2 flowers showed normal FM determinacy and serious organ polarity defect in all floral organs, while arf3-29 only show organ polarity defect in gynoecium. One or two sepal, petals and stamens were removed for better view. Scale bars, 0.5mm.

Figure 1. Floral meristem (FM) determinacy and organ polarity phenotype of Ler (A), arf3-29 (B), kan1 kan2 (C), ag-10 (D), ag-10 arf3-29 (E) and ag-10kan1 kan2 (F). ag-10 arf3-29 displayed FM indeterminacy phenotype showing bulged gynoecium (marked by red arrow). kan1 kan2 and ag-10 kan1 kan2 flowers showed normal FM determinacy and serious organ polarity defect in all floral organs, while arf3-29 only show organ polarity defect in gynoecium. One or two sepal, petals and stamens were removed for better view. Scale bars, 0.5mm.

Figure 2. RNA-seq analysis of ARF3 regulated genes in floral meristem development and gynoecium development. ARF3 up-regulated genes (A) and down-regulated genes (B) in early flower development and gynoecium development were analyzed. The numbers of altered genes are indicated in the pie chart.

Figure 2. RNA-seq analysis of ARF3 regulated genes in floral meristem development and gynoecium development. ARF3 up-regulated genes (A) and down-regulated genes (B) in early flower development and gynoecium development were analyzed. The numbers of altered genes are indicated in the pie chart.

Figure 3. ARF3 mediates the drought stress response during early flower development. (A) The expression of drought stress related genes after ARF3 induction measured by real-time RT-PCR. (B) The gene expression of indicated genes in Ler and arf3-29 under the drought stress condition. Three biological replicates were performed. Error bars represent SD from three biological repeats. *P < 0.05 and **P < 0.01.

Figure 3. ARF3 mediates the drought stress response during early flower development. (A) The expression of drought stress related genes after ARF3 induction measured by real-time RT-PCR. (B) The gene expression of indicated genes in Ler and arf3-29 under the drought stress condition. Three biological replicates were performed. Error bars represent SD from three biological repeats. *P < 0.05 and **P < 0.01.
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