Piriformospora indica promotes the growth of the in-vitro-raised Cymbidium aloifolium plantlet and their acclimatization

Figures & data

Figure 1. In-vitro plant growth assay of Cymbidium aloifolium colonized by P. indica (a) compared to uncolonized plantlets, control (b) for 45 days of growth assay.

Figure 2. Morphological changes in growth pattern of the P. indica-colonized plantlet versus uncolonized plantlet in terms of mean of roots and shoots number (a) as well as mean of root and shoot length (b). Bar represents mean ± SE (n = 15). The data is significant at the level of P ≤ 0.05.

Figure 3. Microscopic view showing the formation of clamdospore in the cortical section of root tissue (arrow indicates the clamdospore formation inside the cortex region of root section).

Table 1. Bioactive compound present in methanolic extracts of the control plantlets (uncolonized).

Peak	Retention time	Name	Mass peak	Base peak	Reported biological function
1.	10.410	Hexadecanoic acid, methyl ester	640	74.10	Antimicrobial activity^Citation17
2.	12.930	Hexadecanoic acid, 1-(hydroxymethyl)-1,2-ethanediyl ester	588	57.15	Antimicrobial activity^Citation17
3.	13.580	D-Mannitol, 1-O-(22-hydroxydocosyl)-	613	73.10	Role in abiotic and biotic stress^Citation18
4.	17.850	Ethyl iso-allocholate	685	55.10	Apoptotic activity^Citation19
5.	25.975	Beta-carotene	820	55.10	Plant biological proecess such as photosynthesis and antioxidant acitvitities

Table 2. Bioactive compound present in methanolic extracts of the P. indica-colonized plantlets.

Peak	Retention time	Compound name	Mass peak	Base peak	Reported biological function
1.	6.333	DL-proline, 5-0xo-,methyl ester	604	84.10	Drought-stress tolerant^Citation20,Citation21
2.	7.400	Hexadecanoic acid, methyl ester	601	74.10	Antimicrobial activity^Citation17
3.	9.985	1H-Indole-3-acetic acid, methyl ester	644	130.15	Plant growth and development, root initiation and elongation^Citation14,Citation15,Citation22
4.	10.715	l-(+)-Ascorbic acid	637	73.10	Important factor in biochemical pathway, biological processes such as photosynthesis^Citation23,Citation24
5.	19.895	Andrographolide	702	107.15	Metabolites having anticancer and antioxidant activities.^Citation25–Citation27

Figure 4. Acclimatization and hardening of the P. indica-colonized plants and uncolonized plants for 3 months using different compositions of substrate at different ratios. (a) Control. (b)Treated. (c) Control. (d) Treated. (e) Control. (f) Treated. (g) Control. (h) Treated.

Figure 5. Morphological changes in growth pattern of P. indica-colonized plantlet versus uncolonized plantlet (3-month-old) after acclimatization in terms of mean of roots and shoots number (a) as well as mean of root and shoot length (b). Bar represents mean ± SE (n = 15). The data is significant at the level of P ≤ 0.05.

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