CbADH1 improves plant cold tolerance

Figures & data

Figure 1. Relative expression level of CbADH1 in homozygous seedlings. Data presented are the mean values of triplicate independent experiments ± SD, and asterisks indicate the significant differences in the expression level compared to WT.

(*p < .05, **p < .01, t-test, Tukey)².

Figure 2. Effect of freezing temperature on electrolyte leakage from the transgene lines and WT plants under (a) non-acclimation condition (NA) and (b) cold acclimation (CA) at 4 °C for 7 d. Data are the mean values of triplicate independent experiments ± SD, and asterisks indicate the significant differences compared to WT.

(*p < .05, **p < .01, t-test, Tukey).²

Figure 3. CbADH1 conferred enhanced cold-shock resistance in Arabidopsis. (a) The photograph showing 28-d-old of transgenic Arabidopsis and WT plants in the growth chamber. (b) Recovery photograph of transgene Arabidopsis and WT in cold shock treatment at −10 °C for 3 h and recovery for 7 d. (c) The survival rate of different transgene plants after recovery for 7 d. Data are the mean values of triplicate independent experiments ± SD, and asterisks indicate the significant differences.

(*p < .05, **p < .01, t-test, Tukey).

Figure 4. CbADH1 conferred enhanced cold-shock resistance in N. benthamiana. (a) The photograph showing 28-d-old of transgenic tobacco and WT plants in the growth chamber. (b) Recovery photograph of transgene tobacco and WT in cold shock treatment at −4 °C for 3 h and recovery for 7 d. (c) The survival rate of different transgene plants after recovery for 7 d. Data are the mean values of triplicate independent experiments ± SD, and asterisks indicate the significant differences.

(*p < .05, **p < .01, t-test, Tukey).

Liu L, Song Y, Xu J, Li D, Li GAn L. Differential expression by chromatin modifications of alcohol dehydrogenase 1 of Chorispora bungeana in cold stress. Gene. 2017;636:1–16. doi:10.1016/j.gene.2017.09.015.

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