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Addendum

Regulatory-Associated Protein of TOR 1B (RAPTOR1B) regulates hormonal switches during seed germination in Arabidopsis thaliana

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Article: 1613130 | Received 22 Mar 2019, Accepted 26 Apr 2019, Published online: 06 May 2019

Figures & data

Figure 1. Delayed germination of raptor1b seeds.

A. raptor1b seeds have altered dormancy. Germination rate was scored from freshly harvested, non-stratified seeds. B. Germination of fully after-ripened WT and raptor1b seeds with or without stratification treatment. C. External application of nitrate did not improve the germination of raptor1b seeds. D. raptor1b seeds showed delayed germination with or without external supplementation of sucrose. Error bars indicate mean ± SD for 5 biological replicates. Black asterisks indicate significantly different from WT (P< .001, Student’s t-test). Stratification refers to seeds that have been kept for 3 days at 4°C. Values of germination percentages are from at least 5 biological replicates of 100 seeds each (100 seeds/plate/5 replicates).

Figure 1. Delayed germination of raptor1b seeds.A. raptor1b seeds have altered dormancy. Germination rate was scored from freshly harvested, non-stratified seeds. B. Germination of fully after-ripened WT and raptor1b seeds with or without stratification treatment. C. External application of nitrate did not improve the germination of raptor1b seeds. D. raptor1b seeds showed delayed germination with or without external supplementation of sucrose. Error bars indicate mean ± SD for 5 biological replicates. Black asterisks indicate significantly different from WT (P< .001, Student’s t-test). Stratification refers to seeds that have been kept for 3 days at 4°C. Values of germination percentages are from at least 5 biological replicates of 100 seeds each (100 seeds/plate/5 replicates).

Figure 2. Quantification of hormones in raptor1b and WT seeds.

Error bars indicate mean ± SD for 5 biological replicates. Black asterisks indicate significantly different from WT (*P< .05, **P< .01, ***P< .001, Student’s t-test).

Figure 2. Quantification of hormones in raptor1b and WT seeds.Error bars indicate mean ± SD for 5 biological replicates. Black asterisks indicate significantly different from WT (*P< .05, **P< .01, ***P< .001, Student’s t-test).

Figure 3. RAPTOR1B positively regulates seed germination in Arabidopsis thaliana.

Figure 3. RAPTOR1B positively regulates seed germination in Arabidopsis thaliana.
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