Figures & data
Figure 1. Accumulation of momilactones A and B in rice leaves. (A) Metabolite content measured in rice leaves by LC-MS/MS after 96 h infestation with BPH. Asterisks indicate statistically significant differences between treatment and control determined by Student’s t-test (*P < .05). n = 5–8; error bars = SEM; FM, fresh mass. (B) Momilactone A and B contents in rice leaves treated with raw or processed HD (filtered, 0.22 μM; or heat treated, 100°C, 20 min) determined by LC-MS/MS after 72 h treatment. Different letters show statistically significant differences between treatments by ANOVA (P < .05; Tukey HSD test). n = 3; error bars = SEM; FM, fresh mass; (C) Momilactone A and B contents in leaves after wounding with a serrated pattern wheel and applying microbial isolates from HDCitation14 suspended in 15% (w/v) sucrose adjusted to OD600 = 0.2. Metabolite levels were determined every 24 h after treatment by LC-MS/MS. Sucrose and HD were used as negative and positive controls, respectively. Different letters show statistically significant differences between treatments at the same time point determined by ANOVA (P < .05; Tukey HSD test). n = 4; error bars = SEM; W+ Suc, wounding with 15% sucrose; W+ Suc+2–08, wounding with isolate 2–08 suspended in 15% sucrose; W+ Suc+4–24, wounding with isolate 4–24 suspended in 15% sucrose; W+ HD, wounding with raw BPH honeydew; W, wounding; Suc, sucrose; HD, honeydew; FM, fresh mass.
![Figure 1. Accumulation of momilactones A and B in rice leaves. (A) Metabolite content measured in rice leaves by LC-MS/MS after 96 h infestation with BPH. Asterisks indicate statistically significant differences between treatment and control determined by Student’s t-test (*P < .05). n = 5–8; error bars = SEM; FM, fresh mass. (B) Momilactone A and B contents in rice leaves treated with raw or processed HD (filtered, 0.22 μM; or heat treated, 100°C, 20 min) determined by LC-MS/MS after 72 h treatment. Different letters show statistically significant differences between treatments by ANOVA (P < .05; Tukey HSD test). n = 3; error bars = SEM; FM, fresh mass; (C) Momilactone A and B contents in leaves after wounding with a serrated pattern wheel and applying microbial isolates from HDCitation14 suspended in 15% (w/v) sucrose adjusted to OD600 = 0.2. Metabolite levels were determined every 24 h after treatment by LC-MS/MS. Sucrose and HD were used as negative and positive controls, respectively. Different letters show statistically significant differences between treatments at the same time point determined by ANOVA (P < .05; Tukey HSD test). n = 4; error bars = SEM; W+ Suc, wounding with 15% sucrose; W+ Suc+2–08, wounding with isolate 2–08 suspended in 15% sucrose; W+ Suc+4–24, wounding with isolate 4–24 suspended in 15% sucrose; W+ HD, wounding with raw BPH honeydew; W, wounding; Suc, sucrose; HD, honeydew; FM, fresh mass.](/cms/asset/e011e3bb-15e1-42ac-b0b6-c8c6dbe759b6/kpsb_a_1655335_f0001_b.gif)