Different regulations of cell-type transcription by UV-B in multicellular green alga Volvox carteri

Figures & data

Table 1. General information regarding RNA-Seq data for both reproductive and somatic cells of V. carteri under UV-B treated or control conditions.

Cell Type	Treatment	Replicate	Sample Name	Raw Reads (Million)	Clean Reads (Million)	Filtered Reads (%)	Mapped Reads (%)
Gonidia	UVB	1	GUVB-A	46.7	46.0	1.49	96.4
	UVB	2	GUVB-B	46.3	45.6	1.51	95.8
	UVB	3	GUVB-C	44.3	43.6	1.58	95.3
	White	1	GW-A	40.8	40.2	1.47	96.4
	White	2	GW-B	40.7	40.2	1.22	96.0
	White	3	GW-C	57.6	56.7	1.56	95.2
Somatic	UVB	1	SUVB-A	41.2	40.4	1.94	95.9
	UVB	2	SUVB-B	28.3	27.0	4.59	95.4
	UVB	3	SUVB-C	51.0	50.3	1.37	94.9
	White	1	SW-A	36.6	36.1	1.36	95.8
	White	2	SW-B	65.4	64.6	1.22	78.6

Figure 1. (a) The number of differentially up- and down-regulated gene features between treatment (UV-B) and control (WL) groups in somatic cells. Note. N is the total number of gene features with and New represents the number of unannotated gene features; (b) Differentially expressed genes (i.e., 0–1, 1–2, & >2 log2 fold change) in somatic cells.

Figure 2. The volcano plot of the transcriptome in somatic cells under UV-B and WL treatment.

Note. The genes above the horizontal line are significantly based on a threshold (FDR<0.05) and the two-fold change threshold (|log2FoldChange|>1), respectively.

Figure 3. Heatmap (log RPKM+1) showing the expression profiles of differentially expressed genes between UV-B treated (UVB) and white light control (w) groups in somatic cells.

Note. Due to non-stranded library to generate the data, some loci included two known genes that are marked with integer number postfixes on the graph. These loci are as below:

Figure 4. Functional analysis of DEGs between the UV-B treatment and control groups in the somatic cells based on gene ontology. GO category was presented for two major functional categories, namely biological process and molecular function.