https://orcid.org/0000-0001-6169-2504
Figures & data
Figure 1. Wilting of ics is overcome by spraying with 1,4-dihydroxy-2-naphthoic acid (NA). Four 18-day-old ics plants sprayed with 0.1 mM NA or buffer (0.1% dimethyl sulfoxide, 0.1% Tween-20) every other day from day 7 after sowing. WT, Golden Promise. Homozygous ics plants were selected by genotyping using the allele-specific primers, ICS-F 5ʹ-TGGCCACAAGGAGCAGTAG-3ʹ, ics-F 5ʹ-GTGGCCACAAGGAGCAGTAA-3ʹ, and reverse primer, ICS-R 5ʹ-TCACTGAACTCAACCTGCAAA-3ʹ, in a touch-down PCR reaction in which the annealing temperature was reduced from 74ºC to 66ºC over the first eight cycles.
Figure 2. Phylloquinone, but not SA, is reduced in ics. Analyses were made by UHPLC-MS/MS according to Glauser et al.Citation20 Leaves from homozygous ICS and ics plants were selected by analyzing DNA from leaf tips as in . Each sample consisted of two first leaves from 10-day-old barley plants. Error bars, SE; n, 6 (phylloquinone) and 8 (SA).
Figure 3. ics has a normal interaction with the powdery mildew fungus. Two days after inoculation with the virulent Bgh isolate, DH14, leaf tips were cut for genotyping (see ), and the remaining leaf parts were trypan blue stained for microscopy. Five leaves (repeats) were assessed from each genotype, and the outcome of 110 to 210 single spore interactions was scored from each leaf according to Zhang et al.Citation21 Error bars, SE.
