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Plant Signaling & Behavior Volume 14, 2019 - Issue 12
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Short communication

Differential distributions of trafficking and signaling proteins of the maize ER-Golgi apparatus

Ikenna O. OkekeogbuDepartment of Botany & Plant Pathology, Purdue University, West Lafayette, IN, USA;Department of Biological Sciences, Purdue University, West Lafayette, IN, USAORCID Iconhttps://orcid.org/0000-0002-2215-2545View further author information
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Uma K. AryalPurdue Proteomics Facility, Bindley Biosciences Center, Purdue University, West Lafayette, IN, USA;Department of Comparative Pathobiology, College of Veterinary Medicine, Purdue University, West Lafayette, IN, USAORCID Iconhttps://orcid.org/0000-0003-4543-1536View further author information
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Susana M. González Fernández-NiñoJoint BioEnergy Institute, Lawrence Berkeley National Laboratory, Berkeley, California, USAORCID Iconhttps://orcid.org/0000-0002-2135-4428View further author information
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Bryan W. PenningUSDA-ARS, Corn, Soybean and Wheat Quality Research, Wooster, OH, USAORCID Iconhttps://orcid.org/0000-0002-0374-8693View further author information
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Joshua L. HeazlewoodJoint BioEnergy Institute, Lawrence Berkeley National Laboratory, Berkeley, California, USAORCID Iconhttps://orcid.org/0000-0002-2080-3826View further author information
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Maureen C. McCannDepartment of Biological Sciences, Purdue University, West Lafayette, IN, USA;Purdue Center for Plant Biology, Purdue University, West Lafayette, IN, USAORCID Iconhttps://orcid.org/0000-0001-6956-4216View further author information
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Nicholas C. CarpitaDepartment of Botany & Plant Pathology, Purdue University, West Lafayette, IN, USA;Department of Biological Sciences, Purdue University, West Lafayette, IN, USA;Purdue Center for Plant Biology, Purdue University, West Lafayette, IN, USACorrespondence[email protected]
ORCID Iconhttps://orcid.org/0000-0003-0770-314XView further author information
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Article: 1672513 | Received 25 Aug 2019, Accepted 20 Sep 2019, Published online: 28 Sep 2019

Figures & data

Table 1. Maize signaling and trafficking proteins identified in ER- and Golgi membranes from etiolated maize coleoptiles.

Figure 1. Relative distribution of ER-Golgi-associated proteins across four major fractions recovered after FFE. Three fractions from FFE were pooled; fractions 27–29 were pooled to give F28, and so forth. (a). Relative abundance of proteins estimated by Absorbance at 280 nm. (b). Relative abundance of proteins most abundant in F28. (c). Relative abundance of proteins most abundant in F34 or F37. (d). Relative abundance of proteins most abundant in F31. Nomenclature as in . Methods used for flotation centrifugation, FFE, and proteomics analysis are described in Reference 1. The MS proteomics data have been deposited to the ProteomeXchange Consortium via the partner repository,Citation20 with the data set identifier PXD007612 and null.

Figure 1. Relative distribution of ER-Golgi-associated proteins across four major fractions recovered after FFE. Three fractions from FFE were pooled; fractions 27–29 were pooled to give F28, and so forth. (a). Relative abundance of proteins estimated by Absorbance at 280 nm. (b). Relative abundance of proteins most abundant in F28. (c). Relative abundance of proteins most abundant in F34 or F37. (d). Relative abundance of proteins most abundant in F31. Nomenclature as in Table 1. Methods used for flotation centrifugation, FFE, and proteomics analysis are described in Reference 1. The MS proteomics data have been deposited to the ProteomeXchange Consortium via the partner repository,Citation20 with the data set identifier PXD007612 and null.

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