https://orcid.org/0000-0002-9284-7318
Figures & data
Figure 1. UV-B inhibited the root growth of Arabidopsis. Wild type (Col-0) was grown under continuous white light or white light with UV-B for 6 d. (a) UV-B inhibition of the root growth of Arabidopsis seedlings; (b) the root length of Arabidopsis under white light or white light with UV-B; WL: White light; WL+UV-B: White light with UV-B, scale bar = 1 mm. Data are expressed as mean values ± standard errors from three replicates, and error bars represent standard errors. The symbol ‘*’ indicates statistical difference P < 0.05, and the symbol ‘****’ indicates statistical difference P < 0.0001 (paired t test, P < 0.05).
Figure 2. UV-B radiation inhibited cell division and cell length. Wild type (Col-0) was grown under continuous white light or white light with UV-B for 6 d. (a) The meristem zone and maturation zone of roots were stained by PI and then visualized by microscopically. (b) The average meristem cell number and cell length of the maturation zone were measured by Image J software, scale bar = 100 μm. Data are expressed as mean values ± standard errors from three replicates, and error bars represent standard errors. The symbol ‘*’ indicates statistical difference P < 0.05 and the symbol ‘****’ indicates statistical difference P < 0.0001 (paired t test, P < 0.05).
Figure 3. Effect of hormones on Arabidopsis root growth. Wild type (Col-0) was grown under continuous white light or white light with UV-B for 6 d. (a) The root length of Arabidopsis with the treatment of GA. (b) The root length of Arabidopsis with the treatment of IAA. (c) The root length of Arabidopsis with the treatment of 6-BA. Data are expressed as mean values ± standard errors from three replicates, and error bars represent standard errors. The symbol ‘*’ indicates statistical difference P < 0.05 and the symbol ‘****’ indicates statistical difference P < 0.0001 (paired t test, P < 0.05).
Figure 4. The primary root length of sto and cop1-4 mutants under UV-B radiation. WT (Col-0), cop1-4, and sto mutants were grown under continuous white light or white light with UV-B for 6 d. (a) Phenotype of primary root length of wild type (Col-0), sto, and cop1-4 mutant. (b) Bar graph showing the primary root length measured by Image J software, scale bar = 1 mm. (c) The ratio (WL+UV-B)/(WL) of root length. Data are expressed as mean values ± standard errors from three replicates, and error bars represent standard errors. The symbol ‘*’ indicates statistical difference P < 0.05 and the symbol ‘****’ indicates statistical difference P < 0.0001 (two-way ANOVA, Turkey’s multiple comparisons test, P < 0.05).
Figure 5. The function of COP1 and STO genes on root cell division and cell length. WT (Col-0), cop1-4, and sto mutants were grown under continuous white light or white light with UV-B for 6 d. Cell number and cell length were measured by ImageJ software. (a) Cell number in meristem zone of root; (b) cell length in mature zone of the root. Data are expressed as mean values ± standard errors from three replicates, and error bars represent standard errors. The symbol ‘*’ indicates statistical difference P < 0.05 and the symbol ‘****’ indicates statistical difference P < 0.0001 (two-way ANOVA, Turkey’s multiple comparisons test).
Figure 6. UV-B-induced root growth inhibition. Wild type (Ler) and uvr8-2 were grown under continuous white light or white light with UV-B for 6 d. The root length was measured by Image J software. Data are expressed as mean values ± standard errors from three replicates, and error bars represent standard errors. The symbol ‘*’ indicates statistical difference P < 0.05 and the symbol ‘****’ indicates statistical difference P < 0.0001 (two-way ANOVA, Turkey’s multiple comparisons test).
