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Tomato seeds pretreated with Antifreeze protein type I (AFP I) promotes the germination under cold stress by regulating the genes involved in germination process

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Article: 1682796 | Received 08 Sep 2019, Accepted 16 Oct 2019, Published online: 24 Oct 2019

Figures & data

Figure 1. Difference in structure between antifreeze proteins type I and type III. This figure is copyrighted by CapicciottiCitation24

Figure 1. Difference in structure between antifreeze proteins type I and type III. This figure is copyrighted by CapicciottiCitation24

Figure 2. Comparison of the size of seedlings germinated from tomato seeds treated with AFP I (100, 300, and 500 µg/l) and control (0 µg/l).

Figure 2. Comparison of the size of seedlings germinated from tomato seeds treated with AFP I (100, 300, and 500 µg/l) and control (0 µg/l).

Figure 3. Comparison of germination percentages of tomato seeds treated with or without antifreeze proteins type I (a) and type III (b) under the growing condition (at 4°C for 5 d, followed by 10°C for 7 d and 20°C for 8 d. Control indicates the seeds soaked only in water. Data represent means of the replications, and bars indicate errors of three replications.

Figure 3. Comparison of germination percentages of tomato seeds treated with or without antifreeze proteins type I (a) and type III (b) under the growing condition (at 4°C for 5 d, followed by 10°C for 7 d and 20°C for 8 d. Control indicates the seeds soaked only in water. Data represent means of the replications, and bars indicate errors of three replications.

Figure 4. Transcript levels of Plasma membrane H+-ATPase in seedlings germinated from the seeds grown in the normal growing condition (at 25°C) and those treated with or without antifreeze proteins type I (a) and type III (b) under low temperature condition (at 4°C for 5 d, followed by 10°C for 7 d and 20°C for 4 d. Control indicates the seeds soaked only in water and exposed to low temperature, and 25°C indicates the seeds soaked only in water and grown in the normal growing condition (25°C) for 16 days. Data represent means of three biological replicates, and bars indicate errors of three biological replicates.

Figure 4. Transcript levels of Plasma membrane H+-ATPase in seedlings germinated from the seeds grown in the normal growing condition (at 25°C) and those treated with or without antifreeze proteins type I (a) and type III (b) under low temperature condition (at 4°C for 5 d, followed by 10°C for 7 d and 20°C for 4 d. Control indicates the seeds soaked only in water and exposed to low temperature, and 25°C indicates the seeds soaked only in water and grown in the normal growing condition (25°C) for 16 days. Data represent means of three biological replicates, and bars indicate errors of three biological replicates.

Figure 5. Transcript level of antioxidant-related genes SOD (a and b) and CAT1 (c and d) expressed in seedlings germinated from the seeds grown in the normal growing condition (at 25°C) and those treated with or without antifreeze proteins type I and III under the low temperature condition (at 4°C for 5 d, followed by 10°C for 7 d and 20°C for 4 d. Control indicates the seeds soaked only in water and exposed to low temperature, and 25°C indicates the seeds soaked only in water and grown in the normal growing condition (25°C) for 16 days. Data represent means of the biological replicates, and bars indicate errors of three biological replicates.

Figure 5. Transcript level of antioxidant-related genes SOD (a and b) and CAT1 (c and d) expressed in seedlings germinated from the seeds grown in the normal growing condition (at 25°C) and those treated with or without antifreeze proteins type I and III under the low temperature condition (at 4°C for 5 d, followed by 10°C for 7 d and 20°C for 4 d. Control indicates the seeds soaked only in water and exposed to low temperature, and 25°C indicates the seeds soaked only in water and grown in the normal growing condition (25°C) for 16 days. Data represent means of the biological replicates, and bars indicate errors of three biological replicates.
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