https://orcid.org/0000-0002-9900-5885
Figures & data
Figure 1. Constitutive expression of GmPSKγ1 promotes growth of vegetative organs and seeds in transgenic Arabidopsis. (a) Schematic representation of the GmPSKγ1 constitutive expression constructs. The GmPSKγ1 CDS, in both full-length and truncated forms, was constructed under control of enhanced CaMV 35S promoter. (b) Semi-quantitative RT-PCR analyses of GmPSKγ1 transcript abundances in leaves of the indicated transgenic lines. Expression of AtActin2 was used as an internal control. (c) Comparison of above-ground parts of 4-week-old wild-type and the indicated transgenic lines. Scale bar, 2 cm. (d–e) Leaf (the largest one) length (d) and fresh weight (e) of the 4-week-old Arabidopsis plants shown in (c). (f) Eleven-d-old wild-type and transgenic Arabidopsis seedlings grown on vertical MS-agar plates. Scale bar, 1 cm. (g) Length of primary roots of the Arabidopsis seedlings shown in (f). (h) Dry mature seeds of wild-type and the indicated transgenic Arabidopsis lines. Scale bar, 0.5 mm. (i–j) Seed size (i) and 100 seed weight (j) of the mature dry seeds shown in (h). Values represent the mean ± SD from three biological replicates. Different letters indicate significantly different values, P < .01, ANOVA test.
Figure 2. PSK-γ does not influence PTI against Pst DC3000. (a) Treatment of PSK-γ has no effect on expression of PAMP-inducible marker genes. Twelve-d-old wild-type seedlings were treated with 1 μM PSK-γ, or 1 μM PSK-α，or 1 μM randomly arranged pentapeptide (TYQYV)， or buffer without peptide, together with 100 nM flg22, for 12 h. Transcript levels of PAMP-inducible marker genes FRK1 and Chitinase were determined by qRT-PCR. (b) Constitutive expression of GmPSKγ1 does not affect the expression of PAMP-inducible genes. Twelve-d-old seedlings of the indicated genotypes were treated with 100 nM flg22 for 8 h and expression of marker genes were determined by qRT-PCR. In (a) and (b), values represent the mean ± SD of three biological replicates, and normalized against the reference gene AtActin2. (c) Disease symptoms of representative leaves of the indicated genotypes at 3 d post inoculation (dpi) of Pst DC3000. Scale bars, 1 cm. (d) Counting of the bacterial number at 0 and 3 dpi. Different letters indicate significantly different values, P < 0.01, ANOVA test.
