Hydrogen sulfide induces Ca²⁺ signal in guard cells by regulating reactive oxygen species accumulation

Figures & data

Figure 1. Assay of H₂O₂ content and net Ca²⁺ fluxes in Arabidopsis thaliana guard cells. (a) The effect of NaHS on H₂O₂ content in guard cells. 100 μM NaHS were used for various treatments for 20 min. Bar = 10 μm. (b) Quantification of H₂O₂ H₂DCF-DA fluorescence density for (a). The average of the fluorescence of each guard cell was calculated. The data are the mean values ± SE (n = 10). (c) The effect of NaHS and H₂O₂ on Ca²⁺ fluxes in guard cells. The Ca²⁺ influxes in guard cells were measured, and 100 mM NaHS or 10 mM H₂O₂ was used. The negative value indicates influx. The data are the mean values ± SE (n = 3). (d) H₂S induces Ca²⁺ signal in guard cells by regulating protein persulfidation and reactive oxygen species accumulation.^13,14 Within each set of experiments, bars with different letters are significantly different at the P < .05 level (Duncan’s multiple range tests)

Chen SS, Jia HL, Wang XF, Shi C, Wang X, Ma P, Wang J, Wang MJ, Li JS. Hydrogen sulfide positively regulates abscisic acid signaling through persulfidation of SnRK2.6 in guard cells. Mol Plant. 2020;13:732–744. doi:https://doi.org/10.1016/j.molp.2020.01.004.

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Shen J, Zhang J, Zhou M, Zhou H, Cui B, Gotor C, Romero LC, Fu L, Yang J, Foyer CH, et al. Persulfidation-based modification of cysteine desulfhydrase and the NADPH oxidase RBOHD controls guard cell abscisic acid signaling. Plant Cell. 2020;32:1000–1017. doi:https://doi.org/10.1105/tpc.19.00826.

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