https://orcid.org/0000-0002-0038-8063
Figures & data
Figure 1. External supplementation of Ca2+ rescues high-Mg sensitivity of mtp10 mutant in a dose dependent manner (a) The phenotype of Col-0 and mtp10 mutant grown in 1/6 MS medium supplemented with 10 mM MgCl2 and different concentrations of Ca2+ conditions. Seedlings were grown in 1/2 MS medium for 3 days, and then transferred to 1/6 MS medium supplemented with 10 mM MgCl2 and external different concentrations of CaCl2 (0, 0.1, 0.3, 0.5, 1, and 3 mM). Bar = 1 cm. (b,c) Quantification of average primary root length (b) and fresh weight (c) of wild-type Col-0 and mtp10 mutants. Data represent means ± SD of five replicate experiments. Asterisks indicate significant difference between the wild-type Col-0 and mtp10 mutant (Student’s t-test, *P < .05).
Figure 2. Ca2+ deficiency can increase the sensitivity of mtp10 to high-Mg stress (a) The phenotype of Col-0 and mtp10 mutant grown in Ca2+ free 1/6 MS medium supplemented with different concentrations of Mg2+. 3-day-old wild-type Col-0 and mtp10 mutant seedlings were planted on 1/2 MS medium and then transferred to the 1/6-MS (without Ca2+) supplemented with different concentrations of MgCl2 (2, 4 and 6 mM) for another two weeks. Bar = 1 cm. (b,c) Quantification of average primary root length and fresh weight of wild-type Col-0 and mtp10 mutants. Data represent means ± SD of five replicate experiments. Asterisks indicate significant difference between the wild-type Col-0 and mtp10 mutant (Student’s t-test, *P < .05).
Figure 3. Fluorescence microscopy of extracellular Ca2+ in leaves of Col-0 and mtp10 mutant. Col-0 and mtp10 seedlings were grown on 1/2 MS solid plates for 7 days and then cultured hydroponically in 1/6 MS medium (a) or 1/6 MS containing 10 mM MgCl2 (b) for another 3 days. Fully expanded leaves were detached and inserted into 200-μL tubes with their corresponding growth medium in the presence of 200-μM fluorescent dye OGB-5 N. Images of the same leaf was taken at different time points (30 min, 1 h, 3 h, and 5 h) of the OGB-5 N treatment.
Figure 4. Metal ions contents in wild-type Col-0 and mtp10 mutant. 7-day-old seedlings grown on 1/2 MS medium were transferred to 1/6 MS liquid medium, 1/6 MS supplemented with 6 mM MgCl2, or 1/6 MS supplemented with 6 mM MgCl2 and 3 mM CaCl2. Samples were collected after being treated for another 10 days. The Mg (a) and Ca (b) contents were measured by ICP-MS. Data are means ± SD. n = 4. Asterisks indicate significant difference between the wild-type Col-0 and mtp10 mutant (Student’s t-test, *P < .05).
