The phosphorylation status of NONPHOTOTROPIC HYPOCOTYL3 affects phot2-dependent phototropism in Arabidopsis

Figures & data

Figure 1. Effects of NPH3^SA and NPH3^SE mutations on the phototropic responses in the etiolated hypocotyls of nph3 mutants and phot1 nph3 double mutants. (a) Experimental scheme of the investigation of hypocotyl phototropism. Two-day-old etiolated seedlings were irradiated continuously with blue light (BL) at 100 µmol m^–2 s^–1 from the abaxial side of the hook. (b) Time courses of the phototropic responses in the 35Spro:NPH3^WT nph3 #2 (WT in the left panel), 35Spro:NPH3^SA nph3 #26 (SA in the left panel), 35Spro:NPH3^SE nph3 #7 (SE in the left panel), 35Spro:NPH3^WT phot1 nph3 #2 (WT in the right panel), 35Spro:NPH3^SA phot1 nph3 #26 (SA in the right panel), and 35Spro:NPH3^SE phot1 nph3 #7 (SE in the right panel). The data shown are the mean values ± SE from 18–40 seedlings. Asterisks indicate statistically significant differences from the curvatures of WT (*p < 0.05, **p < 0.01).

Figure 2. Localization pattern changes for YFP-NPH3 proteins in the phot1 nph3 mutants in response to blue light irradiation. Two-day-old etiolated seedlings of the 35Spro:YFP-NPH3^SA phot1 nph3 (a, c) and 35Spro:YFP-NPH3^SE phot1 nph3 (b, d) lines were irradiated (c, d: +BL) or not (a, b: -BL) with blue light at 100 µmol m^–2 s^–1 for 6 h. YFP signals were detected under a TCS-SP5 confocal laser scanning microscope (Leica Microsystems), as described previously.¹³ Two representative images are shown. White bar, 25 µm.

Kimura T, Haga K, Nomura Y, Higaki T, Nakagami H, Sakai T. Phosphorylation of NONPHOTOTROPIC HYPOCOTYL3 affects photosensory adaptation during the phototropic response. Plant Physiol. 2021;187:981–995. doi:10.1093/plphys/kiab281

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