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Short Communication

Simultaneous mutations in SMN1 and SUMM2 fully suppress the dwarf and autoimmune phenotypes of Arabidopsis mpk4 mutant

, , ORCID Icon, , ORCID Icon & ORCID Icon
Article: 2046412 | Received 03 Feb 2022, Accepted 21 Feb 2022, Published online: 30 Mar 2022

Figures & data

Figure 1. Shoot morphology of Ler, mpk4-1, mpk4-1smn1-98, mpk4-1summ2-8, and mpk4-1smn1-98summ2-8 mutants. Plants were germinated and grown on GM for 10 d, and then grown in soil at 24°C until 1 month of age. Bar = 5 cm. Data is representative of three biological replicates.

Figure 1. Shoot morphology of Ler, mpk4-1, mpk4-1smn1-98, mpk4-1summ2-8, and mpk4-1smn1-98summ2-8 mutants. Plants were germinated and grown on GM for 10 d, and then grown in soil at 24°C until 1 month of age. Bar = 5 cm. Data is representative of three biological replicates.

Figure 2. (a) Rosette morphology of Ler, mpk4-1, mpk4-1smn1-98, mpk4-1summ2-8, and mpk4-1smn1-98summ2-8 mutants. The plants were germinated and grown in soil at 22°C for 3 weeks. Bars = 2 cm. (b) Rosette size of the plants. Values represent averages from the following replicates (Ler n = 15; mpk4-1 n = 32; mpk4-1smn1-98 n = 4; mpk4-1summ2-8 n = 17; mpk4-1smn1-98summ2-8 n = 29), and the error bars denote standard deviation. Asterisks indicate significant differences (*p < .05, **p < .001) compared to the indicated two genotypes (in Welch’s t-test).

Figure 2. (a) Rosette morphology of Ler, mpk4-1, mpk4-1smn1-98, mpk4-1summ2-8, and mpk4-1smn1-98summ2-8 mutants. The plants were germinated and grown in soil at 22°C for 3 weeks. Bars = 2 cm. (b) Rosette size of the plants. Values represent averages from the following replicates (Ler n = 15; mpk4-1 n = 32; mpk4-1smn1-98 n = 4; mpk4-1summ2-8 n = 17; mpk4-1smn1-98summ2-8 n = 29), and the error bars denote standard deviation. Asterisks indicate significant differences (*p < .05, **p < .001) compared to the indicated two genotypes (in Welch’s t-test).

Figure 3. Trypan blue staining of rosette leaves taken from Ler, mpk4-1, mpk4-1smn1-98, mpk4-1summ2-8, and mpk4-1smn1-98summ2-8 mutants. Seeds were germinated on GM for 10 d and then plants were grown in soil at 22°C for 1 month. Detached leaves were stained. Bars = 200 μm except in mpk4-1 (100 μm). Data are representative of three biological replicates.

Figure 3. Trypan blue staining of rosette leaves taken from Ler, mpk4-1, mpk4-1smn1-98, mpk4-1summ2-8, and mpk4-1smn1-98summ2-8 mutants. Seeds were germinated on GM for 10 d and then plants were grown in soil at 22°C for 1 month. Detached leaves were stained. Bars = 200 μm except in mpk4-1 (100 μm). Data are representative of three biological replicates.

Figure 4. PR1 and PR2 expression levels in Ler, mpk4-1, mpk4-1smn1-98, mpk4-1summ2-8, and mpk4-1smn1-98summ2-8 mutants. Plants were grown in soil at 22°C for 3 weeks. Transcript levels are shown relative to Ler as determined by RT-qPCR using Actin2 as an internal standard. Error bars denote standard deviation (n = 3).

Figure 4. PR1 and PR2 expression levels in Ler, mpk4-1, mpk4-1smn1-98, mpk4-1summ2-8, and mpk4-1smn1-98summ2-8 mutants. Plants were grown in soil at 22°C for 3 weeks. Transcript levels are shown relative to Ler as determined by RT-qPCR using Actin2 as an internal standard. Error bars denote standard deviation (n = 3).
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