https://orcid.org/0000-0002-5499-3138
Figures & data
Figure 1. WOX5 inhibits shoot development. (a) Schematic representation of the study design. (b) Representative phenotype of dexamethasone (DEX)-treated 35S::WOX5-GR Arabidopsis seedlings. Wild-type and 35S::WOX5-GR plants were germinated on Murashige and Skoog (MS) medium supplemented with or without microM DEX, and grown under long-day (LD) conditions for 8 days. Scale bars = 1 mm. DAG, days after germination. (c) Percentage of shoot emergence. Cotyledon expansion was used as a phenotypic marker to evaluate shoot emergence. Statistically significant differences were determined using Student’s t-test (n= 33, ***P < 0.001). (d) Expression profiling of genes involved in SAM development. Total RNA was isolated from shoots of 11-day-old seedlings treated for 3 h with microM DEX or ethanol (EtOH). Transcript accumulation was analyzed by RT-qPCR. The eIF4a gene was used as an internal control. Data represent the mean ± standard error of the mean (SEM). Asterisks indicate statistically significant differences (***P < 0.001; Student’s t-test). (e) Schematic of WUS and WOX5 working model. OC-expressed WUS and QC-expressed WOX5 regulate the specification of SAM and RAM, respectively. In addition to their conserved function in pluripotency acquisition, WUS and WOX5 may also have potential roles in conferring distinct tissue identity. Ectopic activation of WUS inhibits root development genes even in roots and promotes shoot organogenesis from root tissues. In contrast, ectopic activation of WOX5 inhibits shoot development genes in shoots possibly through distinct interacting proteins that define tissue identity. SAM, shoot apical meristem; RAM, root apical meristem.
