https://orcid.org/0000-0002-0118-8899
Figures & data
Figure 1. The expression analysis of two PpGLRs under different concentrations of ABA in P. patens. (a) Phenotypes of wild-type (WT) P. patens cultured on BCD medium supplemented with indicated concentrations of ABA. Three biological replicates were done, and representative pictures are shown. (b and c) Statistical analyses of the area and fresh weight of the gametophyte under indicated amounts of hormone. Three biological replicates were done, and data shown are mean of thirty samples from one replicate and error bars indicate SD. (d) semi-quantitative RT-PCR analysis of the expression of PpGLR1 and PpGLR2 under indicated concentration of hormone treatment. Tubulin was used as a loading control. (e) qRT-PCR analysis of PpGLR1 and PpLEA expression under different concentrations of ABA. Three biological replicates were done and similar gene expression trends are shown. Data from three technical repeats are shown, and error bars represent SD. In B, C, and E, ** indicates significant differences between control and hormone-treated sample (P < .01).
Figure 2. PpGLR1pro::GUS reporter analysis of the PpGLR1 expression under ABA treatments. Gametophytes of the PpGLR1pro::GUS transgenic lines were cultured under the control and indicated concentration of the hormone for fifteen days and collected for the analysis. Representative pictures are shown.
Figure 3. The growth of Ppglr1 mutant was hypersensitive to ABA. (a) The gene structure of PpGLR1 (top) and the sequencing results of the PpGLR1 gene in the Ppglr1 mutant showing an 8 bp deletion which would lead to the premature termination of the PpGLR1 protein translation (bottom). (b) Phenotypes of WT and the Ppglr1 mutant lines under the control and ABA treatment. Gametophytes of WT and Ppglr1 mutants were cultured on BCD medium supplemented with 0 or 5 µM ABA for 7 weeks. Three independent experiments were performed, and representative images are shown. (c and d) Statistical analysis of the diameter and fresh weight of the gametophyte from WT and Ppglr1 mutants under indicated amounts of ABA. Data from thirty samples were analyzed under each treatment, and error bars indicate SD. ** indicates significant differences between WT and Ppglr1 mutant under the same condition (P < .01).
