Polymer–metal complex nanoparticles-containing cisplatin and amphiphilic block copolymer for anticancer drug delivery

Figures & data

Table 1. Molecular weights and compositions of synthesized polymers.

Polymer	Feed ratio	Mn (NMR)^c (g/mol)	Mn (GPC) (g/mol)	PDI (GPC)	PCA (%w) (GPC)	PCA (%w) (NMR)^c	Yield (%)
PCL	5/100^a	2200	3340	1.5	–	–	90
PCA–PCL–PCA	4/100^b	4820	7500	1.6	55	54.3	52

^aCoinitiator/ϵ-caprolactone molar ratio.^bPCL/citric acid molar ratio.^cNMR in d₆-DMSO.

Figure 1 Schematic structure of PCA–PCL–PCA–cisplatin.

Figure 2 Particle size distribution of PCA–PCL–PCA–cisplatin nanoparticles at 25 °C in water measured by DLS.

Figure 3 TEM image of PCA–PCL–PCA–cisplatin nanoparticles.

Table 2. Parameters of the prepared nanoparticles.

Samples	Mean diameter (nm)		Zeta potential (mV)
	By DLS	By TEM
PCA–PCL–PCA^a	83	25	−48
PCA–PCL–PCA–cisplatin	234	45	−20.4

^aRef. [28].

Figure 4 AFM image of PCA–PCL–PCA–cisplatin nanoparticles.

Figure 5 (a) Surface zeta potential of blank PCA–PCL–PCA micelles measured by DLS. (b) Surface zeta potential of PCA–PCL–PCA–cisplatin nanoparticles measured by DLS.

Figure 6 (a) Excitation spectra of pyrene as a function of copolymer concentration at room temperature in water (λ_em = 393) for PCA–PCL–PCA–cisplatin. (b) Plot of the intensity ratio I₃₃₆/I₃₃₃ (from pyrene excitation spectra) vs. log (C) for PCA–PCL–PCA–cisplatin.

Figure 7 Cisplatin release profile from PCA–PCL–PCA–cisplatin nanoparticles in phosphate-buffered saline (PBS, pH 7.4, 0.14 M NaCl) at 37 °C.

Figure 8 Viability of AE cells treated with blank PCA–PCL–PCA micelle at 1 mg mL⁻¹ for 24 h.

Figure 9 MTT assay to measure the cytotoxicity of PCA–PCL–PCA–cisplatin nanoparticles against HeLa cell line in comparison with cell culture media, blank PCA–PCL–PCA micelle and free cisplatin.

Gheybi H, Entezami AA. Nanosized micelles self-assembled from amphiphilic poly(citric acid)–poly(ϵ-caprolactone)–poly(citric acid) copolymers. Polym. Bull. 2013;70:1875–1894.

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