1,394
Views
3
CrossRef citations to date
0
Altmetric
Articles

Synthesis of water-soluble fluorescent polymeric glycoconjugate for the detection of cholera toxin

, , , , & ORCID Icon
Pages 150-158 | Received 20 May 2019, Accepted 01 Aug 2019, Published online: 26 Aug 2019

Figures & data

Scheme 1. Synthetic routes to the monomer 3 and polymer PF-Lac.

Scheme 1. Synthetic routes to the monomer 3 and polymer PF-Lac.

Figure 1. 1H NMR spectra of polymer PF-AcLac (in CDCl3) and polymer PF-Lac (in DMSO-d6).

Figure 1. 1H NMR spectra of polymer PF-AcLac (in CDCl3) and polymer PF-Lac (in DMSO-d6).

Figure 2. IR spectra of monomer 3, polymer PF-AcLac, and polymer PF-Lac.

Figure 2. IR spectra of monomer 3, polymer PF-AcLac, and polymer PF-Lac.

Figure 3. UV–vis absorption and fluorescence spectra of polymer PF-Lac in DMSO and water.

Figure 3. UV–vis absorption and fluorescence spectra of polymer PF-Lac in DMSO and water.

Scheme 2. Schematic representation of fluorescence ‘turn-off’ assay for CTB by PF-Lac.

Scheme 2. Schematic representation of fluorescence ‘turn-off’ assay for CTB by PF-Lac.

Figure 4. Fluorescence spectra of polymer PF-Lac (1.5 × 10–6 M) in the absence and presence of various concentrations of CTB in PBS solution at room temperature. CTB concentrations from top to bottom are 0, 1.0, 2.0, 3.0, 4.0, 5.0, 6.0, 7.0 and 8.0 × 10−8 M (Inset displays the photos of the corresponding solutions of PF-Lac in the absence (A) and presence (B) of CTB (8.0 × 10−8 M) under UV light (365 nm) illumination).

Figure 4. Fluorescence spectra of polymer PF-Lac (1.5 × 10–6 M) in the absence and presence of various concentrations of CTB in PBS solution at room temperature. CTB concentrations from top to bottom are 0, 1.0, 2.0, 3.0, 4.0, 5.0, 6.0, 7.0 and 8.0 × 10−8 M (Inset displays the photos of the corresponding solutions of PF-Lac in the absence (A) and presence (B) of CTB (8.0 × 10−8 M) under UV light (365 nm) illumination).

Figure 5. Fluorescence spectra of polymer PF-Lac, polymer PF-Lac with the addition of BSA, and polymer PF-Lac following the addition of BSA and CTB at room temperature.

Figure 5. Fluorescence spectra of polymer PF-Lac, polymer PF-Lac with the addition of BSA, and polymer PF-Lac following the addition of BSA and CTB at room temperature.
Supplemental material

Supplemental Material

Download PDF (320.5 KB)