Dioscin alleviates aplastic anemia through regulatory T cells promotion

Figures & data

Figure 1. The pancytopenia of AA mice model and Effects of Dioscin in mice.(A) The probability of survavial (n = 3). (B) The molecular structure of dioscin (n = 3). (C) Effects of Dioscin on pancytopenia in AA model mouse. Data were expressed as mean ± SD (n = 3). (D) Bone marrow biopsies were stained with HE in the Control, AA and Dioscin groups (200X) (n = 4). (E) IL-10, IL-35 and TGF-β in the Control, AA and Dioscin groups (n = 3). (a. Control group. b. AA group. c. AA + Dioscin group. **P<0.01 vs. control group. ##P<0.01 vs. AA group).

Table 1. Peripheral blood cell count in mice (n = 10).

Groups	WBC(10^9/L)	RBC(10^12/L)	NET(10^9/L)	HGB(g/L)	PLT(10^9/L)	RET(%)
Control	6.20 ± 0.36	8.70 ± 0.96	1.24 ± 0.26	143.0 ± 11.0	1020.4 ± 90.1	7.93 ± 0.16
AA	1.77 ± 0.32**	7.02 ± 0.72**	0.35 ± 0.11**	120.2 ± 16.1**	615.3 ± 126.2**	1.44 ± 0.17**
AA + DNS	6.83 ± 0.64^##	8.33 ± 1.04^##	1.34 ± 0.15^##	141.1 ± 14.53^##	703.5 ± 122.0^##	3.45 ± 0.24^##

**P<0.01 vs. control group. ##P<0.01 vs. AA group.

Table 2. Weight and BMNC in mice (n = 10).

Groups	weight (g)	BMNC (×10^6/femur)
Control	18.5 ± 0.7	22.85 ± 3.19
AA	13.02 ± 1.2**	10.02 ± 2.12**
AA + DNS	15.03 ± 0.44^#	15.13 ± 1.26^#

**P<0.01 vs. control group. ##P<0.01 vs. AA group.

Figure 2. Effects of Dioscin on Foxp3 in splenic lymphocytes of AA mice. (A) Purification of CD4⁺ CD25⁺ Tregs (n = 3). (B) The proliferation of Tregs (n = 3). (C) The cell viability of Tregs treated with Dioscin at 24 h, 48 and 72 h (n = 3). Data were mean ± SD (*P < 0.05 vs. NC group, ^#P < 0.05 vs. AA model group, **P<0.01 vs. control group. ##P<0.01 vs. AA group).

Figure 3. Effects of Dioscin on surface molecules of Tregs. (A): (a) The proportion of CD152 on Tregs among groups (n = 3). (b) The proportion of CD357 on Tregs among groups (n = 3). (c) The proportion of CD39 on Tregs among groups (n = 3). (d) The proportion of CD73 on Tregs among groups (n = 3). (e) The proportion of perforin on Tregs among groups (n = 3). (B) Level of IL-10, IL-35 and TGF-β in the Control, AA and Dioscin groups secreted by Tregs (n = 3). Data were mean ± SD (*P < 0.05 vs. NC group, ^#P < 0.05 vs. AA model group, **P<0.01 vs. control group. ##P<0.01 vs. AA group).