In vitro proliferation of Lebanese Lemna minor and Lemna gibba on different nutrient media

Figures & data

Table 1. Composition of nutrient solutions utilized for the cultivation of L. minor and L. gibba.

	Mineral concentration
	SH	MS	AAP
Medium composition	[18]	[17]	[19]
KNO3	2.48 M	752 mM	0
NH4H2PO4	260 mM	0	0
NaNO3	0	0	0.305 M
NH4NO3	0	824 mM	0
KH2PO4	0	50 mM	0
K2HPO4.3H2O	0	0	6.13 mM
CaCl2.2H2O	136 mM	120 mM	29.9 mM
MgSO4.7H2O	162 mM	60 mM	60.85 mM
MgCl2.6 H2O	0	0	60 mM
H3BO3	8.1 mM	4 mM	3.07 mM
NaHCO3	0	0	178.8 mM
Na2MoO4.2H2O	41 µM	0.04 mM	30.17 µM
FeCl3.6H2O	0	0	0.761 mM
MnSO4.H2O	5.92 mM	4 mM	0
MnCl2.4H2O	0	0	0.805 mM
ZnSO4.7H2O	0.35 mM	1.2 mM	0
ZnCl2	0	0	0.024 mM
CuSO4.5H2O	80.1 µM	0.004 mM	0
CuCl2.2H2O	0	0	0.070 µM
CoCl2.6H2O	42 µM	0	5.884 µM
KI	0.6 mM	0.2 mM	0
FeNaEDTA	5.39 mM	0	0
Na2EDTA	0.55 µM	0	0.806 mM
Fe(III)EDTA	0	4 mM	0

Figure 1. Mean fronds number and SE of L. minor and L. gibba after 21 days of cultivation on SH, MS and AAP media.

Table 2. Mean values and standard errors of DT of proliferation of L. minor and L. gibba along 21 days of culture.

Species	Media	Day 2	Day 5	Day 7	Day 9	Day 12	Day 14	Day 16	Day 19	Day 21
L. minor	SH	2.5 ± 0.39	2.6 ± 0.08^a	2.7 ± 0.07^a	3.2 ± 0.04^a	3.8 ± 0.07^a	3.7 ± 0.09^a	3.9 ± 0.10^a	4.2 ± 0.08^a	4.4 ± 0.05^a
	MS	2.5 ± 0.31	3 ± 0.14^b	4 ± 0.09^b	5.2 ± 0.11^b	7.8 ± 0.78^b	11.4 ± 1.2^b	0	0	0
	AAP	2.6 ± 0.23	3.1 ± 0.20^b	3.3 ± 0.10^c	4 ± 0.08^c	4.7 ± 0.07^c	4.8 ± 0.06^a	4.4 ± 0.10^b	4.4 ± 0.03^b	4.8 ± 0.03^b
L. gibba	SH	2.5 ± 0.13	2.7 ± 0.23	3.6 ± 0.11^a	4.2 ± 0.10^a	5.1 ± 0.31^a	5.6 ± 0.32^a	6.2 ± 0.17^a	7.1 ± 0.13^a	7.6 ± 0.14^a
	MS	2.4 ± 0.16	2.9 ± 0.10	3.4 ± 0.08^a	4.2 ± 0.10^a	5.5 ± 0.05^b	6.6 ± 0.03^b	0	0	0
	AAP	2.5 ± 0.34	2.6 ± 0.08	2.7 ± 0.06^b	2.9 ± 0.03^b	3.6 ± 0.03^c	3.9 ± 0.01^c	4.4 ± 0.07^b	5.0 ± 0.14^b	5.4 ± 0.02^b

*Significantly different on SH and MS and AAP on day 2, 5, 7, 9, 12, 14, 16, 19, and 21, (P < .05). Superscripts a, b, c indicate homogenous value subsets that significantly differ by ANOVA.

Figure 2. The doubling time (DT in days) of L. minor and L. gibba plants in different nutrient media (SH, MS and AAP) based on frond number after 7 days of culture. Line indicates a DT of 2.5 days according to OECD guidelines [19] for the growth inhibition test for Lemna spp.

Figure 3. Growth index of L. minor and L. gibba on SH, MS and AAP media on day 2, 5, 7, 9, 12, 14, 16, 19 and 21.

Figure 4. Proliferation of L. minor and L. Gibba on different nutrient media (SH, MS and AAP) after 7 days of culture (bar = 5 mm).

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