The utility of DNA nanostructures for drug delivery in vivo

Figures & data

Table 1. Overview of the literature describing DNA nanostructures in animal experiments.

Year	Authors	Animal	Structure	Description	Ref
2011	Bhatia et al.	C. elegans	Icosahedral	Cell-specific uptake	[3]
2012	Liu et al.	Mouse	Tetrahedron	Boosting immune response	[4]
2012	Lee et al.	Mouse	Tetrahedron	Tumoral delivery of siRNA	[5]
2013	Surana et al.	C. elegans	Icosahedral	Assessing structural integrity	[6]
2014	Zhang et al.	Mouse	Three origami structures	Tumoral delivery of chemotherapeutic agent	[7]
2014	Amir et al.	Cockroach	DNA origami nanorobot	Molecular computations	[8]
2014	Perrault et al.	Mouse	Origami octahedron	Encapsulation and assessment of biodistribution	[9]
2015	Jiang et al.	Mouse	Two origami structures	Tumoral delivery of gold nanorods	[10]
2016	Arnon et al.	Cockroach	DNA origami nanorobot	Activation by human brain activity	[11]
2016	Huang et al.	Mouse	Tetrahedron	Tumoral delivery of ruthenium polypyridyl (RuPOP)	[12]
2016	Li et al.	Mouse	Bipyramid	Biodistribution of radiolabeled structure	[13]
2016	Jiang et al.	Mouse	Tetrahedron	Biodistribution of radiolabeled structure	[14]

Figure 1. Graph showing the number of articles published and corresponding number of citations for search terms ‘DNA nanotechnology’ and ‘drug delivery’ in titles, abstracts or keywords (www.scopus.com). Red asterix (*) is placed at 2011, when the first paper containing an animal experiment for a DNA nanostructure was published²⁸. The count for 2016 is from August. Full color available online.

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Liu X, Xu Y, Yu T, et al. A DNA nanostructure platform for directed assembly of synthetic vaccines. Nano Lett. 2012;12(8):4254–4259.

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Lee H, Lytton-Jean AKR, Chen Y, et al. Molecularly self-assembled nucleic acid nanoparticles for targeted in vivo siRNA delivery. Nat Nanotechnol. 2012 Jun;7(6):389–393.

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Surana S, Bhatia D, Krishnan Y. A method to study in vivo stability of DNA nanostructures. Methods. 2013;64(1):94–100.

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Zhang Q, Jiang Q, Li N, et al. DNA origami as an in vivo drug delivery vehicle for cancer therapy. ACS Nano. 2014;8(7):6633–6643.

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Amir Y, Ben-Ishay E, Levner D, et al. Universal computing by DNA origami robots in a living animal. Nat Nanotechnol. 2014 Apr 6;9(5):353–357.

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Perrault SD, Shih WM. Virus-inspired membrane encapsulation of DNA nanostructures to achieve in vivo stability. ACS Nano. 2014 May 27; 8(5):5132–5140.

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Jiang Q, Shi Y, Zhang Q, et al. A self-assembled DNA origami-gold nanorod complex for cancer theranostics. Small. 2015;11(38):5134–5141.

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Arnon S, Dahan N, Koren A, et al. Thought-controlled nanoscale robots in a living host. PLoS One. 2016;11(8):e0161227.

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Huang Y, Huang W, Chan L, et al. A multifunctional DNA origami as carrier of metal complexes to achieve enhanced tumoral delivery and nullified systemic toxicity. Biomaterials. 2016;103:183–196.

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Li J, Jiang D, Bao B, et al. Radiolabeling of DNA bipyramid and preliminary biological evaluation in mice. Bioconjug Chem. 2016;27:905–910. acs.bioconjchem.5b00680.

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Jiang D, Sun Y, Li J, et al. Multiple-armed tetrahedral DNA nanostructures for tumor-targeting, dual-modality in vivo imaging. ACS Appl Mater Interf. 2016;8(7):4378-4384.

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