Figures & data
Figure 1. (Colour online) Absorption spectra of GNP functionalised with citrate in blue and GNP functionalised with PEI in red.
Figure 2. FT-IR spectra of (a) Sodium citrate (b) Gold/citrate and (c) PEI and (d) Gold/PEI.
Figure 3. Dynamic light scattering distribution of (a) GNP/Citrate and (b) GNP/PEI.
Figure 4. Zeta potential distribution of (a) GNP/Citrate and (b) GNP/PEI.
Figure 5. (a) Representation of the topographic image of gold nano particles functionalised with citrate and (b) representation of the topographic image of gold nano particles functionalised with PEI.
Figure 6. (a) Cell viability assay by MTT shows reduction in viability of A549 cells with increase in concentrations of GNP/citrate nanoparticles. No significant effect on cellular viability was observed with GNP/PEI and (b) cell viability assays by MTT shows no significant effect on the viability of MCF-7. No significant reduction in viability observed in GNP/PEI and GNP/citrate on MCF-7.
Figure 7. (Colour online) Dot plots show that treatment of A549 cells with GNP/citrate leads to apoptosis in a concentration-dependent manner. (a) 0.4, (b) 0.8, (c) 1.2 and (d) 1.6 µM. The gradual shifting of the A549 cell population towards the P2 population with increase in the GNP/citrate concentration indicates increase in the percentage of apoptosis. (e) Dot plot for untreated cells used as control and (f) Dot plots show that treatment of A549 cells with GNP/PEI at 1.6 µM concentration.
Figure 8. Representative curve of A549 cell death: percent of Annexin V positive cells, which shows a proportional increase with concentration of GNP/citrate.
Figure 9. (a) 500× image of GNP/PEI-treatedA549 and (b) 2000× image of GNP/PEI-treated cells (c) 500× image of GNP/citrate-treated A549 cells and (d) 2000× image of GNP/citrate-treated cells.
Figure 10. (Colour online) (a) 40× image of the A549 cells treated with GNP/PEI (b) 100× image of the A549 cells treated with GNP/PEI, (c) 40× image of the A549 cells treated with GNP/citrate and (d) 100× image of the A549 cells treated with GNP/Citrate.
Figure 11. (Lane 1) wide-range DNA ladder sequence. (Lane 2) Total genomic DNA from untreated A549 cells. (Lane 3) Total genomic DNA from treated A549 cells.
