Lipopolysaccharide influences on the toxicity of oxidised multiwalled carbon nanotubes to murine splenocytes in vitro

Figures & data

Table 1. Physicochemical properties of the oxidised multiwalled carbon nanotubes (Ox-MWCNT).

Physicochemical properties		Ox-MWCNT
(A)	Diameter (nm)	10–40
(B)	Length (μm)	<5 μm
(C)	Surface area (m^2/g)	250
(D)	Zeta potential (mV)	−26 ± 5.0
(E)	ID/IG	1.38
(F)	Decomposition temperature (ºC)	585
(G)	Qualitative elementary analysis	C, O, Fe
(H)	Metallic residue (iron oxide) (%)	1.2

Figure 1. Transmission electronic microscopy (A) and scanning electronic microscopy (B) of oxidised multiwalled carbon nanotubes (Ox-MWCNT).

Figure 2. Surviving of splenocytes after culture with Ox-MWCNT. Splenocytes were isolated from spleen and 2 × 10⁵ cells cultured with different concentrations of Ox-MWCNT diluted in RPMI media supplemented with 10% FBS. The viability was analysed 24 and 48 h after in Trypan blue exclusion method and 96 h in MTT assay: (A) cell viability determined with Trypan blue; (B) viability determined through MTT assay. *P < 0.05.

Figure 3. Effects of LPS in cytotoxicity of Ox-MWCNT forsplenocytes in vitro: (A) after sterilisation process Ox-MWCNT was washed with LPS-Free water and the residual water used to quantify the LPS. Samples beyond 0.5 EU/mL were considered positive. Splenocytes were isolated from spleen and 2 × 10⁵ cells cultured with 10 ng/mL of Ox-MWCNT diluted in RPMI media supplemented with 10% FBS. The viability was analyzed after 48 h in Trypan blue exclusion method and 96 h for in MTT assay; (B) cell viability determined with Trypan blue; (C) viability determined through MTT assay. *P < 0.05.