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Journal of Experimental Nanoscience Volume 15, 2020 - Issue 1
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Article

Chemical characterization and neuroprotective properties of copper nanoparticles green-synthesized by nigella sativa L. seed aqueous extract against methadone-induced cell death in adrenal phaeochromocytoma (PC12) cell line

Wen Yana Department of Neurology, the First People’s Hospital of Lanzhou, Lanzhou City, China
,
Yutang Liub Department of Neurology, Xi’an TCM Hospital of Encephalopathy, Xi’an, ChinaCorrespondence[email protected]
,
Shirin Mansooridarac Faculty of Medicine, Medical Sciences Research Center, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran
,
Atoosa Shahriyari Kalantarid Faculty of Medicine, Department of Neurology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
,
Nastaran Sadeghiane Faculty of Sciences, Department of Chemistry, Ataturk University, Erzurum, Turkey
,
Parham Taslimif Faculty of Science, Department of Biotechnology, Bartin University, Bartin, Turkey
,
Akram Zangenehg Faculty of Veterinary Medicine, Department of Clinical Sciences, Razi University, Kermanshah, Iran;h Biotechnology and Medicinal Plants Research Center, Ilam University of Medical Sciences, Ilam, Iran
&
Mohammad Mahdi Zangenehg Faculty of Veterinary Medicine, Department of Clinical Sciences, Razi University, Kermanshah, Iran;h Biotechnology and Medicinal Plants Research Center, Ilam University of Medical Sciences, Ilam, Iran
 show all
Pages 280-296 | Received 08 May 2020, Accepted 31 May 2020, Published online: 26 Jun 2020

Figures & data

Figure 1. The image of Nigella sativa L. seed.

Figure 1. The image of Nigella sativa L. seed.

Figure 2. The UV–Vis spectrum of biosynthesized copper nanoparticles.

Figure 2. The UV–Vis spectrum of biosynthesized copper nanoparticles.

Figure 3. FT-IR spectra of biosynthesized copper nanoparticles.

Figure 3. FT-IR spectra of biosynthesized copper nanoparticles.

Figure 4. FE-SEM image of copper nanoparticles.

Figure 4. FE-SEM image of copper nanoparticles.

Figure 5. TEM image of copper nanoparticles.

Figure 5. TEM image of copper nanoparticles.

Figure 6. The cell viability of different treatments after 48 h.

M: Methadone, C: Control, T1: 100μM methadone and 2μg of Cu(NO3)2, T2: 100μM methadone and 4μg of Cu(NO3)2, T3: 100μM methadone and 2μg of N. sativa, T4: 100μM methadone and 4μg of N. sativa, T5: 100μM methadone and 2μg of CuNPs, T6: 100μM methadone and 4μg of CuNPs.

*indicate the significant difference (p ≤ 0.01) between experimental groups with methadone group.

Figure 6. The cell viability of different treatments after 48 h.M: Methadone, C: Control, T1: 100μM methadone and 2μg of Cu(NO3)2, T2: 100μM methadone and 4μg of Cu(NO3)2, T3: 100μM methadone and 2μg of N. sativa, T4: 100μM methadone and 4μg of N. sativa, T5: 100μM methadone and 2μg of CuNPs, T6: 100μM methadone and 4μg of CuNPs.*indicate the significant difference (p ≤ 0.01) between experimental groups with methadone group.

Figure 7. The apoptosis index of different treatments after 48 h.

M: Methadone, C: Control, T1: 100μM methadone and 2μg of Cu(NO3)2, T2: 100μM methadone and 4μg of Cu(NO3)2, T3: 100μM methadone and 2μg of N. sativa, T4: 100μM methadone and 4μg of N. sativa, T5: 100μM methadone and 2μg of CuNPs, T6: 100μM methadone and 4μg of CuNPs.

*indicate the significant difference (p ≤ 0.01) between experimental groups with methadone group.

Figure 7. The apoptosis index of different treatments after 48 h.M: Methadone, C: Control, T1: 100μM methadone and 2μg of Cu(NO3)2, T2: 100μM methadone and 4μg of Cu(NO3)2, T3: 100μM methadone and 2μg of N. sativa, T4: 100μM methadone and 4μg of N. sativa, T5: 100μM methadone and 2μg of CuNPs, T6: 100μM methadone and 4μg of CuNPs.*indicate the significant difference (p ≤ 0.01) between experimental groups with methadone group.

Figure 8. The cytokine concentration in different treatments after 48 h.

M: Methadone, C: Control, T1: 100μM methadone and 2μg of Cu(NO3)2, T2: 100μM methadone and 4μg of Cu(NO3)2, T3: 100μM methadone and 2μg of N. sativa, T4: 100μM methadone and 4μg of N. sativa, T5: 100μM methadone and 2μg of CuNPs, T6: 100μM methadone and 4μg of CuNPs.

*indicate the significant difference (p ≤ 0.01) between experimental groups with methadone group.

Figure 8. The cytokine concentration in different treatments after 48 h.M: Methadone, C: Control, T1: 100μM methadone and 2μg of Cu(NO3)2, T2: 100μM methadone and 4μg of Cu(NO3)2, T3: 100μM methadone and 2μg of N. sativa, T4: 100μM methadone and 4μg of N. sativa, T5: 100μM methadone and 2μg of CuNPs, T6: 100μM methadone and 4μg of CuNPs.*indicate the significant difference (p ≤ 0.01) between experimental groups with methadone group.

Figure 9. The caspase 3 absorption in different treatments after 48 h.

M: Methadone, C: Control, T1: 100μM methadone and 2μg of Cu(NO3)2, T2: 100μM methadone and 4μg of Cu(NO3)2, T3: 100μM methadone and 2μg of N. sativa, T4: 100μM methadone and 4μg of N. sativa, T5: 100μM methadone and 2μg of CuNPs, T6: 100μM methadone and 4μg of CuNPs.

*indicate the significant difference (p ≤ 0.01) between experimental groups with methadone group.

Figure 9. The caspase 3 absorption in different treatments after 48 h.M: Methadone, C: Control, T1: 100μM methadone and 2μg of Cu(NO3)2, T2: 100μM methadone and 4μg of Cu(NO3)2, T3: 100μM methadone and 2μg of N. sativa, T4: 100μM methadone and 4μg of N. sativa, T5: 100μM methadone and 2μg of CuNPs, T6: 100μM methadone and 4μg of CuNPs.*indicate the significant difference (p ≤ 0.01) between experimental groups with methadone group.

Figure 10. The mitochondrial membrane potential of different treatments after 48 h.

M: Methadone, C: Control, T1: 100μM methadone and 2μg of Cu(NO3)2, T2: 100μM methadone and 4μg of Cu(NO3)2, T3: 100μM methadone and 2μg of N. sativa, T4: 100μM methadone and 4μg of N. sativa, T5: 100μM methadone and 2μg of CuNPs, T6: 100μM methadone and 4μg of CuNPs.

*indicate the significant difference (p ≤ 0.01) between experimental groups with methadone group.

Figure 10. The mitochondrial membrane potential of different treatments after 48 h.M: Methadone, C: Control, T1: 100μM methadone and 2μg of Cu(NO3)2, T2: 100μM methadone and 4μg of Cu(NO3)2, T3: 100μM methadone and 2μg of N. sativa, T4: 100μM methadone and 4μg of N. sativa, T5: 100μM methadone and 2μg of CuNPs, T6: 100μM methadone and 4μg of CuNPs.*indicate the significant difference (p ≤ 0.01) between experimental groups with methadone group.

Figure 11. The antioxidant properties of Cu(NO3)2, N. sativa, CuNPs, and BHT against DPPH.

Figure 11. The antioxidant properties of Cu(NO3)2, N. sativa, CuNPs, and BHT against DPPH.

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