Modification of silk fibroin nanofibers scaffold by PAMAM dendrimer for cell culture

Figures & data

Figure 1. Morphologies of SF nanofibers at different concentration: a. 5%; b. 7%; c. 9%; d. 11%; e. 13%; f. 15%.

Figure 2. The schematic of mechanism of crosslinking of SF with PAMAM G2 by EDC-NHS.

Table 1. Concentrations of EDC/NHS and PAMAM for surface modification of SF nanofibers scaffold.

5	4	3	2	1	Sample No.
0.5%	1%	1.5%	2%	2.5%	NHS
1%	2%	3%	4%	5%	EDC
7%	7%	7%	7%	7%	PAMAM

Figure 3. SEM images taken after treatment of SF nanofibers: (a) EDC = 5, NHS = 2.5, (b) EDC = 4, NHS = 2, (c) EDC = 3, NHS = 1.5, (d) EDC = 2, NHS = 1, (e) EDC = 1, NHS = 0.5.

Figure 4. MTT assays and cytotoxicity studies against L-929 Cells: (a) Cell viability (%) During 24 h of incubation, (b) Optical microscopy images after incubation with control, (c) Optical microscopy images after incubation with SF nanofibers scaffold.

Figure 5. FTIR transmittance spectra of: (A) Degummed silk fibroin. (B) Silk fibroin nanofibers scaffold modified by PAMAM dendrimers.

Figure 6. X-ray diffraction patterns of silk fibroins. (A) Pure silk fibroin. (B) Silk fibroin treated with dendrimer.