Figures & data
Figure 1. Schematic comparison of a neuron–sensor interface using a flat sensor and a sensor with needles.
Figure 2. Schematic representation of the PA22-2 immobilisation on gold surfaces.
Figure 3. SEM picture: (a) an array of Au needles and (b) detailed view of a single needle.
Figure 4. Coupling of the peptide on the gold needles of the chip: (a) brightfield image of the chip and (b) fluorescent image of the same area.
Figure 5. Z-stack of TLN expressing HeLa cells cultured on top of Alexa 555 labelled PA22-2 on top of the needles. The small insets form a schematic representation of the experimental set-up with the confocal plane of the picture as a dashed line. The consecutive pictures are taken at 0.5 µm intervals.
Figure 6. SEM pictures of HeLa cells growing on needles: (a) overview picture of a cell growing on a needle-bed. (b) Detailed picture of a cell extension that engulfs several needles.
Figure 7. SEM pictures of PC12 cells growing on PA22-2-coated gold needles: (a) overview of the entire cell as it grows on the needles. (b) Detailed view showing a strong interaction between the cell membrane and the needles that becomes visible through the bending of the needles.
