Nano-aggregates using thermosensitive chitosan copolymers as a nanocarrier for protein delivery

Figures & data

Figure 1. The chemical structure of the CP copolymer.

Figure 2. The schematic route of BSA-loaded CP nano-aggregates.

Table 1. The CAC value and size of the CP nano-aggregates.

Sample	CAC (g L^−1)	Size (nm)		ζ-potential (mV)
		No drug	With BSA
CP1K	0.100	81.6 ± 15.10	158.8 ± 32.80	61.11
CP1.5K	0.063	110.3 ± 21.80	160.0 ± 36.30	60.43
Note: n = 3, mean ± SD.

Figure 3. The size distribution of the BSA-loaded (a) CP1K and (b) CP1.5K nano-aggregates.

Table 2. BSA loading amount of the CP nano-aggregates.

Sample	DLA (µg mg^−1)	DLE (%)
CP1K	107.1 ± 6.68	62.5 ± 0.04
CP1.5K	95.1 ± 2.70	55.5 ± 0.02
Note: n = 3, mean ± SD.

Figure 4. In vitro release profiles of BSA from the CP nano-aggregates in 0.01 M PBS at 37°C: (shaded circle) CP1K and (light shaded square) CP1.5K. Note: n = 3, mean ± SD.