Autologous hematopoietic stem cell grafts - moving away from a one-size-fits-all approach

Figures & data

Table 1. Examples of patient and autologous graft manufacturing process heterogeneity. The multi-step variability will potentially be reflected in graft composition and infused immune cell doses

Patient	Conditioning	Mobilization	HSC collection	Graft preparation
Age	Induction	Chemotherapy	Apheresis device	Concentration/dilution
Weight	Consolidation	Combination therapy	Procedure length	Plasma removal
Diagnosis	Chemotherapy	Mobilizing agents	Centrifugation technique	Additive solutions
Disease risk	Total body irradiation	Formulation	Anticoagulation	Cryoprotectant
Remission	Formulation	Dose	Additives	Cryopreservation
Comorbidity	Dose/schedule/duration	Schedule	Processed blood volume	Storage conditions
Overall risk	Toxicity	Duration	Number of collections	Storage duration

Figure 1. Methods for optimizing autologous hematopoietic stem cell grafts. Autologous hematopoietic stem cell grafts are influenced by several factors and the graft may be affected qualitatively and quantitatively by several factors including stem cell mobilization approaches, collection and storages techniques, and direct methods for preparation and improvement of the graft (Figure made by BioRender).