The macroscopic and microscopic effects of imidazolium ionic liquids on blood and their interactions with serum albumins

Figures & data

Figure 1. (a) Blood clotting index, (b) hemolysis rate, (c) hemoglobin concentration, (d) conductivity and (e) serum protein concentration of blood with [Bmim]Br, [Bmim]Cl, [Bmim]BF₄ and [Bmim]PF₆.

Figure 2. (a) Blood clotting index, (b) hemolysis rate, (c) hemoglobin concentration, (d) conductivity and (e) serum protein concentration of blood with [Emim]PF₆, [Bmim]PF₆ and [Omim]PF₆.

Figure 3. Erythrocyte morphology (a) without the IL and that treated with (b) [Bmim]PF₆ and (c) [Omim]PF₆; (d) erythrocyte sedimentation (±0.01 mm) of blood with ionic liquids ① [Emim]PF₆, ② [Bmim]PF₆, ③ [Omim]PF₆ and ④ without IL; thrombolytic rate of (e) ILs (10 mg/mL) at different time, and (f) different [Emim]PF₆ dose in 2 mL PBS at 3 h.

Figure 4. (a) UV spectra with different [Bmim]BF₄ concentrations; (b) fluorescence spectra with different [Bmim]BF₄ concentrations and the fluorescence of the combined solution without or with 10 μM [Bmim]BF₄ under 280 nm; (c) IR spectra of RSA with different [Bmim]BF₄ concentrations; (d) the absorbance of the complex formed by the mixture of [Bmim]BF₄ and two kinds of serum albumin at 278 nm; molecular docking diagram of the interactions between [Bmim]BF₄ (green, blue, pink and cyanic sticks) and (e) RSA amino acid residues (yellow fragments) or (f) HSA amino acid residues (cyanic fragments).