Digestibility of Juncus acutus and its effects on ruminal cellulolytic bacteria

Figures & data

Table 1. Chemical composition of Juncus acutus, % unless otherwise stated.

Constituents	Station I	Station II	Station III
	X ± SX (n = 20)	X ± SX (n = 20)	X ± SX (n = 20)
DM	97.68 ± 0.23	97.99 ± 0.24	97.78 ± 0.26
ASH	4.02 ± 0.03	4.10 ± 0.02	4.21 ± 0.01
OM	93.66 ± 0.09	93.89 ± 0.13	93.57 ± 0.08
CP	10.03 ± 0.06^a	9.79 ± 0.07^b	9.77 ± 0.07^b
EE	1.47 ± 0.06	1.48 ± 0.03	1.46 ± 0.02
NDF	73.66 ± 0.10	73.70 ± 0.08	73.70 ± 0.10
ADF	46.08 ± 0.04	46.58 ± 0.11	46.17 ± 0.10
ADL	12.27 ± 0.06^a	12.17 ± 0.09^b	12.01 ± 0.12^c
MEADF, MJ/kg DM	8.61 ± 0.01	8.55 ± 0.02	8.60 ± 0.01

DM, dry matter; OM, organic matter; CP, crude protein; EE, ether extract; NDF, neutral detergent fibre; ADF, acid detergent fibre; ADL, acid detergent lignin; ME_ADF, Metabolisable energy calculated from ADF.

a,b,c Means within a row with different superscripts differ (p< 0.05).

Table 2. Cumulative gas production volume at 24 h (GP), potential gas production volume (b), organic matter digestibility (OMD) and metabolisable energy (ME_OMD and ME _GP) of Juncus acutus.

Parameters	Station I	Station II	Station III
	X ± SX (n=20)	X ± SX (n=20)	X ± SX (n=20)
Ppsi	7.39 ± 0.16^a	7.15 ± 0.19^ab	6.79 ± 0.14^b
GPmL	18.31 ± 0.41^a	17.73 ± 0.47^ab	16.87 ± 0.39^b
OMD, %	42.64 ± 0.02^a	42.48 ± 0.15^b	42.54 ± 0.14^b
MEOMD, MJ/kg DM	6.82 ± 0.02^a	6.79 ± 0.02^b	6.81 ± 0.02^b
MEGP, MJ/kg DM	5.26 ± 0.05^a	5.17 ± 0.06^ab	5.05 ± 0.05^b
b, mL	21.1078 ± 0.2606^ab	20.4587 ± 0.2700^b	21.9427 ± 0.4543^a
c, mL/h	0.0950 ± 0.0053^a	0.0832 ± 0.0038^b	0.0548 ± 0.0031^c
T1/2, h	7.29 ± 0.13^c	8.33 ± 0.16^b	12.65 ± 0.18^a

b, potential gas production (mL); c, the gas production rate constant for the insoluble fraction (mL/h); T_1/2, the time taken to produce the half of the total gas pool (h).

a,b,c Means within a row with different superscripts differ (p< 0.05).

Table 3. Fibrobacter succinogenes, Ruminococcus flavefaciens and Ruminococcus albus values in rumen fluids obtained from 0, 3, 6, 12, 24, 48, 72 and 96 h incubations of Juncus acutus samples which were collected from three different stations.

T	F. succinogenes				R. flavefaciens				R. albus
	Sta I	Sta II	Sta III	SEM	Sta I	Sta II	Sta III	SEM	Sta I	Sta II	Sta III	SEM
0	1	1	1		1	1	1		1	1	1
3	1.13^a	1.04^a	1.23^b	0.03	1.05	1.03	1.07	0.02	1.03	1.04	1.03	0.01
6	1.31^b	1.30^b	1.48^a	0.05	1.23^a	1.10^b	1.19^a	0.03	1.05	1.07	1.04	0.03
12	1.63^b	1.85^a	1.89^a	0.03	1.32	1.37	1.37	0.04	1.14	1.23	1.23	0.02
24	2.96^a	2.73^b	2.75^b	0.06	2.29^b	2.41^a	1.94^c	0.05	1.90^a	1.64^b	1.59^b	0.04
48	3.90^a	3.58^b	3.70^b	0.05	2.69^a	2.67^a	2.53^b	0.03	2.29	2.23	2.32	0.05
72	3.90^a	3.68^b	3.70^b	0.08	2.71^a	2.69^a	2.58^b	0.04	2.31	2.28	2.37	0.04
96	3.94^a	3.68^b	2.68^b	0.06	2.71^a	2.73^a	2.60^b	0.04	2.29	2.29	2.39	0.03

SEM, mean of standard error; Sta, Station; T, incubation time (h).

a,b,c Means within a row with different superscripts differ (p< 0.05).