Influence of partial substitution of dietary marine origin feedstuffs by a mixture of extruded pea seed meal and animal origin feedstuffs on fatty acid composition of fillet in sea bass (Dicentrarchus labrax)

Figures & data

Table 1. Ingredients (%) and cost (?/100 kg) of the experimental diets.

	Diets
	TD	MD
Fish meal (herring)	15	30
Anchovy oil	10	10
Krill meal	–	5
Soybean oil	1	–
Peas meal	9
Soybean meal	12	12
Rapeseed meal	10	10
Sunflower meal	10	10
Wheat	5	5
Gluten	11	11
Feather meal	5
Spray-dried blood	5	–
Premix^a	7	7
Cost of the diets (€/100 kg)	82	98

TD: terrestrial diet; MD: marine diet.^aSupplied the following mg/kg feed (?-tocopherol, 185.0; Thiamin, 12.0; Riboflavin, 20.0; Niacin, 45.0; Pyridoxal phosphate, 11.0; Menadione, 10.0; Biotin, 0.35; Folic acid, 15.0; Ascorbate phosphate, 105.0; Zn, 102.0; I, 1.4; Mg, 30.0; Co, 1.0).

Table 2. Proximate analysis (% dry basis) and selected fatty acid composition (g/100g of total fatty acid methyl esters) of the diets.

	Diets
	TD	MD
Analysed chemical composition
Crude protein	39.9	40.1
Crude lipids	14.9	15.0
Ash	7.2	7.6
Main individual methyl esters fatty acids^a
C16:0	12.69	15.25
C18:1 n-9	26.95	19.90
C18:2 n-6	11.87	9.05
C18:3 n-3	3.33	1.82
C20:4 n-6	0.65	0.69
C20:5 n-3	3.51	8.44
C22:6 n-3	7.85	10.10

TD: terrestrial diet; MD: marine diet.^aMain FA selected from a list of identified FA on the chromatograms.

Table 3. Proximate composition of the fillets of sea bass fed the experimental diets.

	Dietary treatment
	TD	MD	SEM	Significance
Number of fish	10	10	10
Dry matter, %	27.35	28.79	0.536	ns
Ash, %	1.28	1.26	0.104	ns
Lipids, %	3.83	4.41	0.481	ns
Crude protein, %	20.09	19.88	0.300	ns

TD: terrestrial diet; MD: marine diet; ns: not significant; SEM: standard error of means.

Table 4. Growth parameters of the fish during the experiment.

	Dietary treatment
	TD	MD	SEM
IBW, g	30.2	30.8	3.6
FBW, g	324.4	332.3	9.9
^aWG, g	294.2	301.5	9.6
^bFCR	1.9	1.9	0.1

There were no significant differences between diets.

TD: terrestrial diet; MD: marine diet; SEM: standard error of the mean; IBW: initial body weight; FBW: final body weight.

a WG, weight gain = FBW − IBW.

b FCR, feed conversion ratio =feed consumption/WG.

Table 5. Identified fatty acids of the fillets of sea bass fed the experimental diets (g/100 g of total fatty acid methyl esters).

	Dietary treatment
	TD	MD	SEM	Significance
Number of fish	10	10	10
C12:0	0.04	0.04	0.001	ns
C14:0	2.36	3.11	0.124	p < .05
C16:0	15.26	15.82	0.189	ns
C16:1 n-7	3.57	4.38	0.142	p < .05
C17:0	0.28	0.32	0.008	ns
C17:1	0.44	0.58	0.023	p < .05
C18:0	3.52	3.69	0.051	ns
C18:1 n-9	29.63	23.36	1.020	p < .001
C18:2 n-6	11.08	9.16	0.313	p < .001
C18:3 n-6	0.20	0.20	0.005	ns
C18:3 n-3	2.61	1.61	0.158	p < .001
C20:0	0.27	0.25	0.004	ns
C20:1	0.28	0.34	0.010	ns
C20:3 n-6	2.07	2.11	0.026	ns
C20:3 n-3	0.58	0.61	0.013	ns
C20:4 n-6	0.69	0.79	0.023	p < .05
C22:0	0.37	0.47	0.016	ns
C20:5 n-3	4.14	6.39	0.359	p < .001
C20:1 n-9	0.90	1.13	0.038	ns
C22:5 n-3	1.08	1.42	0.058	p < .001
C22:6 n-3	8.95	9.72	0.169	p < .01
Unknown	11.49	14.52	0.706	ns
SFA	22.37	24.04	0.329	p < .01
MUFA	37.21	32.31	0.836	p < .001
PUFA	31.40	32.01	0.242	ns
∑ n − 3	17.36	19.75	0.407	p < .001
∑ n − 6	14.04	12.26	0.300	p < .001
∑n-6/∑n-3	0.81	0.62	0.030	p < .01

TD: terrestrial diet; MD: marine diet; ns: not significant; SEM: standard error of means; SFA: saturated fatty acids; MUFA: monounsaturated fatty acids; PUFA: polyunsaturated fatty acids.