Influence of γ-glutamyltransferase and alkaline phosphatase activity on in vitro fertilisation of bovine frozen/thawed semen

Figures & data

Table 1. Enzymatic activities (U/L) in semen samples.

	Sperm cells			Supernatant
	Mean ± SE	Min	Max	Mean ± SE	Min	Max
GGT	575.2 ± 50.1^A	80.0	1032.1	2884.7 ± 262.8^B	727.6	5229.8
ALP	1739.6 ± 177.0^a	639.8	3959.1	2779.4 ± 380.7^b	714.1	8732.7

^a,b Values with different superscripts are significantly different; p < .05.

^A,B Values with different superscripts are significantly different; p < .001.

Table 2. Percentages of motility, viability, cleavage, blastocysts and GGT and ALP activities in sperm cells (SC) and surnatant (S) in bovine frozen-thawed semen samples (means of the 3 ejaculates per 8 bull).

Bulls	Motility Mean ± SE	Viability Mean ± SE	Cleavage Mean ± SE	Blastocysts Mean ± SE	GGT (SC) Mean ± SE	ALP (SC) Mean ± SE	GGT (S) Mean ± SE	ALP (S) Mean ± SE
1	46.3 ± 2.7	70.1 ± 3.5	67.0 ± 4.2	20.8 ± 6.7	34.5 ± 8.4	136.6 ± 45.5	165.9 ± 32.9	232.0 ± 50.3
2	37.0 ± 3.8	81.2 ± 2.3	76.7 ± 2.9	31.0 ± 2.8	27.4 ± 1.8	76.3 ± 10.0	109.6 ± 10.5	121.9 ± 33.4
3	43.3 ± 4.5	74.2 ± 9.0	72.8 ± 5.8	25.4 ± 4.1	34.7 ± 5.3	118.6 ± 12.6	106.7 ± 14.7	250.6 ± 76.9
4	47.3 ± 3.2	78.1 ± 3.1	70.7 ± 5.7	29.0 ± 7.5	25.2 ± 8.7	128.9 ± 12.1	103.5 ± 5.3	94.6 ± 23.6
5	36.7 ± 3.4	75.3 ± 3.8	71.2 ± 7.3	18.2 ± 7.4	20.2 ± 3.6	81.6 ± 14.8	68.0 ± 4.1	165.9 ± 38.3
6	36.3 ± 3.8	59.7 ± 5.6	71.1 ± 1.0	24.5 ± 1.3	19.1 ± 2.5	56.1 ± 14.9	75.5 ± 22.8	44.5 ± 8.3
7	42.3 ± 3.7	76.3 ± 1.7	68.7 ± 2.0	22.2 ± 6.1	28.2 ± 8.0	77.1 ± 27.8	128.4 ± 37.1	240.8 ± 91.7
8	38.3 ± 5.2	68.2 ± 3.8	72.5 ± 3.4	25.9 ± 0.2	28.0 ± 6.8	51.9 ± 7.7	93.7 ± 19.8	73.0 ± 25.0